利用真菌生物高效合成银纳米颗粒及其对[具体对象1]和[具体对象2]的抗真菌功效

The Biogenically Efficient Synthesis of Silver Nanoparticles Using the Fungus and Their Antifungal Efficacy against and .

作者信息

El-Ashmony Ranya M S, Zaghloul Nouf S S, Milošević Marija, Mohany Mohamed, Al-Rejaie Salim S, Abdallah Yasmine, Galal Anwar A

机构信息

Department of Plant pathology, Minia University, Elminya 61519, Egypt.

Bristol Centre for Functional Nanomaterials, HH Wills Physics Laboratory, Tyndall Avenue, University of Bristol, Bristol BS8 1FD, UK.

出版信息

J Fungi (Basel). 2022 Jun 2;8(6):597. doi: 10.3390/jof8060597.

DOI:10.3390/jof8060597

PMID:35736080

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9225611/

Abstract

Silver nanoparticles (AgNs) are known as a promising alternative tool to control fungal diseases. AgNs were biologically synthesized using as an ecofriendly approach. The presence of AgNs was confirmed by changing the color to brown, followed by UV-Vis spectroscopy, transmission electron microscopy (TEM), and Energy-dispersive spectra (EDS). TEM studies showed that the size of AgNs average was 31.13 nm and the shape was spherical. In vitro assays of AgNs showed a significant inhibitory effect on the growth of () and (). The percentage inhibition on mycelial linear growth, dry weight, and sclerotia formation of and at 100 were 87.8, 82.7, 96.4, 52.8, 55.1, and 85.4%, respectively. The obtained results suggested that the biosynthesized AgNs have antifungal activity against and S. . Foliar spray of bean and sunflower plants with AgNs caused a decrease in disease severity, which promoted the plant protection against and , respectively. Substantially, this study will extend our understanding of the AgNs antifungal action for suppressing fungal diseases.

摘要

银纳米颗粒（AgNs）被认为是控制真菌病害的一种有前景的替代工具。采用生物合成法制备AgNs是一种环境友好型方法。通过颜色变为棕色，随后利用紫外可见光谱、透射电子显微镜（TEM）和能量色散谱（EDS）证实了AgNs的存在。TEM研究表明，AgNs的平均尺寸为31.13 nm，形状为球形。AgNs的体外试验表明，其对立枯丝核菌（Rhizoctonia solani）和核盘菌（Sclerotinia sclerotiorum）的生长具有显著抑制作用。在100 μg/mL浓度下，AgNs对立枯丝核菌和核盘菌的菌丝线性生长、干重及菌核形成的抑制率分别为87.8%、82.7%、96.4%、52.8%、55.1%和85.4%。所得结果表明，生物合成的AgNs对立枯丝核菌和核盘菌具有抗真菌活性。用AgNs对豆类和向日葵植株进行叶面喷施可降低病害严重程度，分别促进了植株对立枯丝核菌和核盘菌的抗性。实质上，本研究将扩展我们对AgNs抑制真菌病害抗真菌作用的理解。