酶联免疫吸附测定（ELISA）。

Enzyme-Linked Immunosorbent Assay (ELISA).

机构信息

Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada.

Faculty of Sustainable Design Engineering, University of Prince Edward Island, Charlottetown, PE, Canada.

出版信息

Methods Mol Biol. 2022;2508:115-134. doi: 10.1007/978-1-0716-2376-3_10.

DOI:10.1007/978-1-0716-2376-3_10

PMID:35737237

Abstract

Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA assay, and clearly outline protocols for different types of ELISA assays, including direct, indirect, sandwich, competitive, and nanoparticle-based ELISA.

摘要

酶联免疫吸附测定（ELISA）是研究和临床中检测生物分子最特异和直接的方法之一。随着分析方法的进步，ELISA 检测不断得到优化以提高其灵敏度，现在有不同类型的 ELISA 可用于检测各种生物标志物。本章全面总结了 ELISA 的基本原理，讨论了 ELISA 检测的不同组成部分，并详细介绍了不同类型 ELISA 检测的方案，包括直接 ELISA、间接 ELISA、夹心 ELISA、竞争 ELISA 和基于纳米颗粒的 ELISA。