Castiglioni V, Vailati Facchini R, Mattiello S, Luini M, Gualdi V, Scanziani E, Recordati C
Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria (DIPAV), Facoltà di Medicina Veterinaria, via Celoria 10, 20133 Milano, Italy.
Vet Microbiol. 2012 Sep 14;159(1-2):107-14. doi: 10.1016/j.vetmic.2012.03.026. Epub 2012 Mar 27.
Enterohepatic Helicobacter spp. have been described colonizing the large intestine and liver of healthy and symptomatic subjects and are thought to have a role in the development of inflammatory bowel disease (IBD). The prevalence of enterohepatic Helicobacter spp. infection in dogs is largely unknown and to our knowledge there are no data about their potential pathogenic role. In light of these considerations, the aims of this study were (i) to assess the prevalence of enterohepatic Helicobacter spp. in colonic biopsies of symptomatic pet dogs and (ii) to evaluate a possible association between Helicobacter spp. colonization status (heavily colonized, poorly colonized and uncolonized biopsies) and histological lesions. Colonic biopsies from 27 pet dogs of different ages were evaluated by family Helicobacteraceae and enterohepatic Helicobacter spp. PCR, histology, and immunohistochemistry for the in situ detection of Helicobacter spp. organisms. 85% and 52% of colonic biopsies were positive by Helicobacteraceae and enterohepatic Helicobacter spp. PCR, respectively. Immunohistochemistry revealed Helicobacter spp. were localized both in the superficial mucus (55%) and within intestinal crypts (33%). Dogs with heavy enterohepatic Helicobacter spp. colonization were significantly younger and had a higher level of mucosal fibrosis/atrophy than dogs with uncolonized or poorly colonized biopsies (p<0.05). These findings contribute to widen current knowledge regarding canine enterohepatic Helicobacter spp., suggesting the infection is rather common in dogs and acquired at an early age. Furthermore, heavy colonization of colonic crypts is associated with chronic inflammatory lesions (fibrosis/atrophy), supporting the role of enterohepatic Helicobacter spp. in the development of canine IBD.
肝肠螺杆菌属已被描述为可在健康和有症状个体的大肠和肝脏中定殖，并被认为在炎症性肠病（IBD）的发生发展中起作用。肝肠螺杆菌属感染在犬类中的患病率很大程度上未知，据我们所知，尚无关于其潜在致病作用的数据。鉴于这些考虑因素，本研究的目的是：（i）评估有症状宠物犬结肠活检中肝肠螺杆菌属的患病率；（ii）评估螺杆菌属定殖状态（重度定殖、轻度定殖和未定殖活检）与组织学病变之间的可能关联。通过幽门螺杆菌科和肝肠螺杆菌属PCR、组织学以及免疫组织化学对27只不同年龄宠物犬的结肠活检进行评估，以原位检测螺杆菌属微生物。分别有85%和52%的结肠活检通过幽门螺杆菌科和肝肠螺杆菌属PCR检测呈阳性。免疫组织化学显示，螺杆菌属既定位于表层黏液（55%），也定位于肠隐窝内（33%）。与活检未定殖或轻度定殖的犬相比，肝肠螺杆菌属重度定殖的犬明显更年轻，黏膜纤维化/萎缩水平更高（p<0.05）。这些发现有助于拓宽目前关于犬肝肠螺杆菌属的知识，表明该感染在犬类中相当常见且在幼年时获得。此外，结肠隐窝的重度定殖与慢性炎症病变（纤维化/萎缩）相关，支持肝肠螺杆菌属在犬IBD发生发展中的作用。
