Iqbal Muhammad Shahid, Tang Shurong, Sarfraz Zareen, Iqbal Muhammad Sajid, Li Hongge, He Shoupu, Jia Yinhua, Sun Gaofei, Pan Zhaoe, Xiaoli Geng, Mahmood Abid, Ahmad Saghir, Nazir Mian Faisal, Chen Baojun, Wang Liru, Pang Baoyin, Wei Shoujun, Du Xiongming
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences (ICR, CAAS), Anyang, China.
Ayub Agricultural Research Institute Faisalabad, Cotton Research Institute, Multan, Pakistan.
Front Genet. 2021 Dec 7;12:758665. doi: 10.3389/fgene.2021.758665. eCollection 2021.
The study of A-genome Asian cotton as a potential fiber donor in Gossypium species may offer an enhanced understanding of complex genetics and novel players related to fiber quality traits. Assessment of individual fibers providing classified fiber quality information to the textile industry is Advanced Fiber Information System (AFIS) in the recent technological era. Keeping the scenario, a diverse collection of 215 Asiatic cotton accessions were evaluated across three agro-ecological zones of China. Genome-Wide Association Studies (GWAS) was performed to detect association signals related to 17 AFIS fiber quality traits grouped into four categories viz: NEPs, fiber length, maturity, and fineness. Significant correlations were found within as well as among different categories of various traits related to fiber quality. Fiber fineness has shown a strong correlation to all other categories, whereas these categories are shown interrelationships fiber-fineness. A total of 7,429 SNPs were found in association with 17 investigated traits, of which 177 were selected as lead SNPs. In the vicinity of these lead SNPs, 56 differentially expressed genes in various tissues/development stages were identified as candidate genes. This compendium connecting trait-SNP-genes may allow further prioritization of genes in GWAS loci to enable mechanistic studies. These identified quantitative trait nucleotides (QTNs) may prove helpful in fiber quality improvement in Asian cotton through marker-assisted breeding as well as in reviving eroded genetic factors of . introgression breeding.
将A基因组亚洲棉作为棉属潜在纤维供体进行研究，可能有助于更深入地了解与纤维品质性状相关的复杂遗传学和新的相关因素。在当今技术时代，为纺织工业提供分类纤维质量信息的单纤维评估方法是先进纤维信息系统（AFIS）。基于此，在中国的三个农业生态区对215份亚洲棉种质资源进行了多样性评估。开展了全基因组关联研究（GWAS），以检测与17个AFIS纤维品质性状相关的关联信号，这些性状分为四类，即：短纤维含量、纤维长度、成熟度和细度。在与纤维品质相关的不同类别性状内部以及之间均发现了显著相关性。纤维细度与所有其他类别均表现出强相关性，而这些类别之间也呈现出与纤维细度的相互关系。共发现7429个单核苷酸多态性（SNP）与17个研究性状相关，其中177个被选为主要SNP。在这些主要SNP附近，鉴定出56个在不同组织/发育阶段差异表达的基因作为候选基因。这种将性状-SNP-基因联系起来的纲要可能有助于进一步确定GWAS位点中的基因优先级，以开展机制研究。这些已鉴定的数量性状核苷酸（QTN）可能有助于通过标记辅助育种改善亚洲棉的纤维品质，以及在渐渗育种中恢复已流失的遗传因子。
