通过微量凝集试验和微量酶联免疫吸附测定检测军团菌病病原体抗体。
Detection of antibodies to legionnaires disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests.
作者信息
Farshy C E, Klein G C, Feeley J C
出版信息
J Clin Microbiol. 1978 Apr;7(4):327-31. doi: 10.1128/jcm.7.4.327-331.1978.
Abstract
Microagglutination and micro-enzyme-linked immunosorbent assay (ELISA) tests with easily prepared, safe, heat-killed antigens for detecting antibodies to the legionnaires disease organism have been developed. A safranin-stained whole-cell antigen is used in the microagglutination test, and a simply prepared soluble antigen is used in the micro-ELISA tests. The microagglutination test detected elevated titers in 97.2% of the sera from patients with legionnaires disease. Three variations of the micro-ELISA test with anti-human immunoglobulin G, immunoglobulin M, and Fab peroxidase-labeled conjugates revealed elevated titers with 74.3, 82.9, and 88.6% of the sera, respectively. The microagglutination and the micro-ELISA tests used in combination detected 100% of the elevated titers.
摘要
已开发出用于检测军团菌病病原体抗体的微量凝集试验和微量酶联免疫吸附测定（ELISA）试验，所用抗原易于制备、安全且经热灭活。微量凝集试验使用番红染色的全细胞抗原，微量ELISA试验使用简单制备的可溶性抗原。微量凝集试验在97.2%的军团菌病患者血清中检测到滴度升高。使用抗人免疫球蛋白G、免疫球蛋白M和Fab过氧化物酶标记结合物的三种微量ELISA试验变体，分别在74.3%、82.9%和88.6%的血清中检测到滴度升高。联合使用微量凝集试验和微量ELISA试验可检测到100%的滴度升高。
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