Cho Sohyun, McMillan Elizabeth A, Barrett John B, Hiott Lari M, Woodley Tiffanie A, House Sandra L, Frye Jonathan G, Jackson Charlene R
Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA-ARS, U.S. National Poultry Research Center, Athens, GA 30605, USA.
Oak Ridge Institute for Science and Education, Oak Ridge, TN 37830, USA.
Microorganisms. 2022 Jun 17;10(6):1244. doi: 10.3390/microorganisms10061244.
The presence and transfer of plasmids from commensal bacteria to more pathogenic bacteria may contribute to the dissemination of antimicrobial resistance. However, the prevalence of plasmids from commensal bacteria, such as the enterococci, in food animals remains largely unknown. In this study, the diversity and prevalence of plasmid families from multidrug-resistant (MDR; resistance to three or more antimicrobials) enterococci from poultry carcasses were determined. Plasmid-positive MDR enterococci were also tested for the ability to transfer plasmids to other enterococci using conjugation. MDR ( = 98) and ( = 696) that were isolated from poultry carcass rinsates between 2004 and 2011 were tested for the presence of 21 plasmid replicon () families using multiplex PCR. Approximately 48% of (47/98) and 16% of (110/696) were positive for at least one -family. Fourteen -families were detected overall, and ten -families were shared between and . The and families were unique to , while the and families were unique to . The family was predominant in both and for all the years tested. The greatest number of -families detected was in 2005 ( = 10), and the least was in 2009 ( = 1). Eight -families were transferred from donors to the JH2-2 recipient using conjugation. Results from this study showed that and from poultry carcasses contain numerous and diverse -families that are capable of conjugal transfer.
共生细菌中质粒的存在及其向致病性更强的细菌的转移可能会促进抗菌药物耐药性的传播。然而，诸如肠球菌等共生细菌中的质粒在食用动物中的流行情况仍 largely 未知。在本研究中，测定了来自家禽屠体的耐多药（MDR；对三种或更多种抗菌药物耐药）肠球菌的质粒家族的多样性和流行情况。还使用接合试验检测了质粒阳性的 MDR 肠球菌将质粒转移至其他肠球菌的能力。对 2004 年至 2011 年间从家禽屠体冲洗液中分离出的 MDR（n = 98）和非 MDR（n = 696）菌株，使用多重 PCR 检测 21 种质粒复制子（rep）家族的存在情况。约 48%的 MDR（47/98）和 16%的非 MDR（110/696）至少对一种 rep 家族呈阳性。总共检测到 14 种 rep 家族，其中 10 种 rep 家族在 MDR 和非 MDR 之间共有。Inc18 和 Inc22 家族是 MDR 特有的，而 Inc1 和 Inc9 家族是非 MDR 特有的。在所有检测年份中，IncF 家族在 MDR 和非 MDR 中均占主导。检测到的 rep 家族数量最多的是 2005 年（n = 10），最少的是 2009 年（n = 1）。使用接合试验将 8 种 rep 家族从 MDR 供体转移至非 MDR JH2 - 2 受体。本研究结果表明，来自家禽屠体的 MDR 和非 MDR 含有大量多样的可进行接合转移的 rep 家族。
