Wang Chia-Hsien, Mutalik Chinmaya, Yougbaré Sibidou, Teng Nai-Chia, Kuo Tsung-Rong
Division of Prosthodontic Dentistry, Department of Dentistry, Taipei Medical University Hospital, Taipei 11031, Taiwan.
International Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
Nanomaterials (Basel). 2022 Jun 10;12(12):1997. doi: 10.3390/nano12121997.
The artificial repair of tooth enamel is still an urgent requirement because it has a complicated and well-arranged structure. Herein, calcium phosphate nanoclusters (CaP NCs) were synthesized, via a facile approach, for application in the repair of tooth enamel erosion. Structural and optical characterizations validated the successful preparation of spherical CaP NCs, with an average size of 2.1 ± 0.11 nm. By evaporating the ethanol and triethylamine (TEA) solvents, pure CaP was produced, which was further used to repair the tooth enamel. Simulated caries lesions were achieved via phosphoric acid etching to cause damage to enamel rods. After repair, the damaged enamel rods were directly covered with CaP. According to microhardness testing, after repair with CaP NCs, the hardness value of the tooth enamel with acid etching increased to a similar level to that of normal tooth enamel. The results of the microhardness test indicated that CaP NCs revealed great potential for repairing tooth enamel erosion. Our work demonstrates a promising potential for treating the early stage of tooth erosion with CaP NCs. Based on these findings, we believe that stable CaP NCs can be employed as a precursor for the tunable, effective repair of tooth enamel in the near future.
由于牙釉质具有复杂且排列有序的结构，其人工修复仍然是一项迫切需求。在此，通过一种简便的方法合成了磷酸钙纳米团簇（CaP NCs），用于牙釉质侵蚀修复。结构和光学表征证实成功制备了平均尺寸为2.1±0.11 nm的球形CaP NCs。通过蒸发乙醇和三乙胺（TEA）溶剂，制备出纯CaP，并进一步用于修复牙釉质。通过磷酸蚀刻模拟龋损病变，对釉柱造成损伤。修复后，受损的釉柱直接被CaP覆盖。根据显微硬度测试，用CaP NCs修复后，经酸蚀刻的牙釉质硬度值增加到与正常牙釉质相似的水平。显微硬度测试结果表明，CaP NCs在修复牙釉质侵蚀方面具有巨大潜力。我们的工作证明了用CaP NCs治疗牙侵蚀早期具有广阔前景。基于这些发现，我们相信在不久的将来，稳定的CaP NCs可作为可调谐、有效修复牙釉质的前体。
