Stockhaus J, Eckes P, Blau A, Schell J, Willmitzer L
Nucleic Acids Res. 1987 Apr 24;15(8):3479-91. doi: 10.1093/nar/15.8.3479.
ST-LS1, a single copy gene from potato displaying a leaf/stem specific gene expression, was tagged by an exon modification and introduced into both potato and tobacco cells using Agrobacterium vectors. After regeneration of whole plants, the expression of the tagged gene was analyzed with respect to its organ specificity and compared to the expression of the corresponding resident gene. The expression of the transferred gene in transgenic plants closely followed the expression of the resident gene. No marked influence of the plant species serving as host was observed. The level of expression of the introduced gene varied by a factor of at least 100 in independent transformants when normalized to the expression of the resident gene. Southern analysis performed on the transformed plants indicated a correlation between copy number of the introduced gene and its expression level. The activity of the tagged gene as well as of the resident gene was significantly inhibited by treatment of the transgenic plants with the herbicide norfluorazon, indicating that this gene activity is dependent on the presence of functional chloroplasts in the leaves.
ST-LS1是来自马铃薯的一个单拷贝基因，具有叶/茎特异性基因表达，通过外显子修饰进行标记，并使用农杆菌载体导入马铃薯和烟草细胞。在再生出完整植株后，分析了标记基因的表达及其器官特异性，并与相应内源基因的表达进行了比较。转基因植物中转移基因的表达与内源基因的表达密切相关。未观察到作为宿主的植物物种有明显影响。当以内源基因的表达进行标准化时，导入基因在独立转化体中的表达水平变化至少100倍。对转化植株进行的Southern分析表明，导入基因的拷贝数与其表达水平之间存在相关性。用除草剂去甲草净处理转基因植株后，标记基因和内源基因的活性均受到显著抑制，这表明该基因活性依赖于叶片中功能性叶绿体的存在。
