Department of Pathology, University of Iowa, Iowa City, IA, USA.
Department of Neuroscience and Pharmacology, University of Iowa, Iowa City, IA, USA.
BMC Res Notes. 2022 Jun 25;15(1):219. doi: 10.1186/s13104-022-06100-5.
Inflammation is present in many diseases and identification of immune cell infiltration is a common assessment. CD138 (syndecan-1) is a recommended immunohistochemical marker for human plasmacytes although it is also expressed in various epithelia and tumors. Similarly, CD138 is a marker for murine plasmacytes, but its tissue immunostaining is not well-defined. Endogenous CD138 expression is an important confounding factor when evaluating plasmacyte infiltration. We studied two plasmacyte markers (CD138 and Kappa light chains) for endogenous immunostaining in five organs and one tumor from B6 mice.
Plasmacytes in Peyer's patches were positive for CD138 and Kappa markers without endogenous immunostaining. Endogenous CD138 immunostaining was widespread in liver, kidney, lung and a malignant peripheral nerve sheath tumor (MPNST) versus regionalized immunostaining in skin and small intestine wall. Endogenous Kappa immunostaining was absent in all tissues except for plasmacytes. Tissues with widespread endogenous CD138 immunostaining were contrasted by absence of endogenous Kappa immunostaining. Here, plasmacytes would not be distinguished by CD138, but would be obvious by Kappa immunostaining. Our study suggests that utility of immunostaining for plasmacytes by CD138 is tissue dependent in mice. Additionally, Kappa immunostaining may be a useful alternative in mouse tissues with confounding endogenous CD138 immunostaining.
炎症存在于许多疾病中，免疫细胞浸润的鉴定是一种常见的评估方法。CD138（连接蛋白-1）是人类浆细胞的一种推荐的免疫组织化学标志物，尽管它也在各种上皮细胞和肿瘤中表达。同样，CD138 是鼠浆细胞的标志物，但它的组织免疫染色尚未得到很好的定义。内源性 CD138 表达是评估浆细胞浸润时的一个重要混杂因素。我们研究了 B6 小鼠的五个器官和一个肿瘤中的两种浆细胞标志物（CD138 和 Kappa 轻链）的内源性免疫染色。
派尔氏淋巴结中的浆细胞对 CD138 和 Kappa 标志物呈阳性，没有内源性免疫染色。肝、肾、肺和恶性外周神经鞘瘤（MPNST）中的内源性 CD138 免疫染色广泛，而皮肤和小肠壁中的免疫染色则局限于局部。除浆细胞外，所有组织均无内源性 Kappa 免疫染色。内源性 CD138 免疫染色广泛的组织与内源性 Kappa 免疫染色缺失形成对比。在这里，CD138 不能区分浆细胞，但 Kappa 免疫染色可以明显区分浆细胞。我们的研究表明，在小鼠中，CD138 用于浆细胞免疫染色的效果依赖于组织。此外，在存在内源性 CD138 免疫染色的混杂的小鼠组织中，Kappa 免疫染色可能是一种有用的替代方法。
