Brewster Wendy R, Burkett Wesley C, Ko Emily M, Bae-Jump Victoria, Nicole McCoy Amber, Keku Temitope O
University of North Carolina at Chapel Hill, Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, United States.
University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, United States.
Gynecol Oncol Rep. 2022 Jun 10;42:101017. doi: 10.1016/j.gore.2022.101017. eCollection 2022 Aug.
The microbiome of the female upper reproductive tract (URT) has not been characterized. We hypothesize that distinct bacterial species may be identified in different areas of the URT in women with or without ovarian cancers.
Postmenopausal women scheduled for salpingooophorectomy were prospectively identified. We excluded those who used antibiotics within three months of surgery or had a diagnosed gynecologic cancer. Bacteria were extracted from tissue samples of the proximal fallopian tube, fimbriae and ovaries of 10 women. Using molecular-phylogenetic methods based on the highly conserved 16S bacteria rRNA gene, we assessed the complexity of URT microbiota in tissue samples by high throughput sequencing of the V1-V3 region of the 16S gene. Sequences were processed through QIIME and an average of 69,625 reads per sample was obtained after quality filtering. Multivariate analyses were conducted using PRIMER VI software.
The initial analysis of samples suggests that bacteria exist in the URT. Analysis of similarity matrix (ANOSIM) suggests that the microbiome differs in the areas examined (ANOSIM R = 0.26, p = 0.015). The microbiome differs significantly between the fallopian tube and ovary (ANOSIM R = 0.23, p = 0.02). The proximal fallopian tube microbiome also differs from the fimbriae (ANOSIM R = 0.66, p = 0.025). There were borderline differences in the microbial profiles of the specimens with and without epithelial ovarian cancer (p = 0.06).
We identified distinct microbiota of the ovaries and fallopian tubes with a profile unique to women with epithelial ovarian cancer. Further investigation is necessary to determine whether the microbiome is related to ovarian carcinogenesis.
女性上生殖道（URT）的微生物群尚未得到充分描述。我们假设，在患有或未患有卵巢癌的女性中，上生殖道不同区域可能存在不同的细菌种类。
前瞻性纳入计划接受输卵管卵巢切除术的绝经后女性。排除那些在手术前三个月内使用过抗生素或已确诊患有妇科癌症的女性。从10名女性的近端输卵管、输卵管伞端和卵巢的组织样本中提取细菌。使用基于高度保守的16S细菌rRNA基因的分子系统发育方法，通过对16S基因V1-V3区域进行高通量测序，评估组织样本中上生殖道微生物群的复杂性。序列通过QIIME进行处理，质量过滤后每个样本平均获得69,625条读数。使用PRIMER VI软件进行多变量分析。
样本的初步分析表明上生殖道中存在细菌。相似性分析矩阵（ANOSIM）表明，所检查区域的微生物群不同（ANOSIM R = 0.26，p = 0.015）。输卵管和卵巢之间的微生物群存在显著差异（ANOSIM R = 0.23，p = 0.02）。近端输卵管微生物群也与输卵管伞端不同（ANOSIM R = 0.66，p = 0.025）。有上皮性卵巢癌和无上皮性卵巢癌的标本的微生物谱存在临界差异（p = 0.06）。
我们鉴定出卵巢和输卵管独特的微生物群，其特征在上皮性卵巢癌女性中是独特的。有必要进一步研究以确定微生物群是否与卵巢癌发生有关。
