Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Sci Rep. 2019 Feb 8;9(1):1691. doi: 10.1038/s41598-018-38031-2.
Ovarian carcinoma is caused by multiple factors, but its etiology associated with microbes and infection is unknown. Using 16S rRNA high-throughput sequencing methods, the diversity and composition of the microbiota from ovarian cancer tissues (25 samples) and normal distal fallopian tube tissues (25 samples) were analyzed. High-throughput sequencing showed that the diversity and richness indexes were significantly decreased in ovarian cancer tissues compared to tissues from normal distal fallopian tubes. The ratio of the two phyla for Proteobacteria/Firmicutes was notably increased in ovarian cancer, which revealed that microbial composition change might be associated with the process of ovarian cancer development. In addition, transcriptome-sequencing (RNA-seq) analyses suggested that the transcriptional profiles were statistically different between ovarian carcinoma and normal distal fallopian tubes. Moreover, a set of genes including 84 different inflammation-associated or immune-associated genes, which had been named as the human antibacterial-response genes were also modulated expression. Therefore, we hypothesize that the microbial composition change, as a novel risk factor, may be involving the initiation and progression of ovarian cancer via influencing and regulating the local immune microenvironment of fallopian tubes except for regular pathways.
卵巢癌是由多种因素引起的，但与微生物和感染相关的病因尚不清楚。本研究采用 16S rRNA 高通量测序方法，分析了 25 例卵巢癌组织（卵巢癌组织）和 25 例正常远端输卵管组织（正常输卵管组织）的微生物群落多样性和组成。高通量测序结果显示，与正常输卵管组织相比，卵巢癌组织中的微生物多样性和丰富度指数显著降低。在卵巢癌中，变形菌门/厚壁菌门的比值明显增加，这表明微生物组成的变化可能与卵巢癌的发展过程有关。此外，转录组测序（RNA-seq）分析表明，卵巢癌和正常远端输卵管之间的转录谱存在统计学差异。此外，还发现了一组包括 84 个不同的炎症相关或免疫相关基因的基因，这些基因被命名为人类抗菌反应基因，它们的表达也受到了调节。因此，我们假设微生物组成的变化作为一个新的危险因素，可能通过影响和调节输卵管的局部免疫微环境，而不是通过常规途径，参与卵巢癌的发生和发展。
