Kong Xiang-Qi, Wang Yong-Jing, Fang Zan-Xi, Yang Tian-Ci, Tong Man-Li
Center of Clinical Laboratory, School of Medicine, Zhongshan Hospital, Xiamen University, Xiamen, China.
Xiamen Clinical Laboratory Quality Control Center, Xiamen, China.
Front Med (Lausanne). 2022 Jun 10;9:847407. doi: 10.3389/fmed.2022.847407. eCollection 2022.
Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard for diagnosing coronavirus disease 2019 (COVID-19). However, RT-PCR may yield false-positive results, leading to unnecessary countermeasures. Here, we report a "positive" nucleic acid test on a 10-pooled sample during the routine screening that caused many adverse societal effects, and financial and resource losses. However, they were subsequently determined to be a case of vaccine contamination. This case study increases awareness of false-positive RT-PCR results for SARS-CoV-2, especially when participants are vaccinators. Moreover, it could provide relevant suggestions to prevent the recurrence of such incidents.
实时逆转录聚合酶链反应（RT-PCR）是诊断2019冠状病毒病（COVID-19）的金标准。然而，RT-PCR可能会产生假阳性结果，从而导致不必要的应对措施。在此，我们报告了一例在常规筛查期间对10合1样本进行的“阳性”核酸检测，该检测造成了许多不良社会影响以及财务和资源损失。然而，这些结果随后被确定为疫苗污染的情况。本案例研究提高了对SARS-CoV-2的RT-PCR假阳性结果的认识，尤其是当参与者为疫苗接种者时。此外，它可以为防止此类事件再次发生提供相关建议。
