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铁线莲属植物叶绿体全基因组测序及其系统发育分析。

Sequencing of the complete chloroplast genome of Diels and its phylogenetic analysis.

作者信息

Wang Wei, Xu Tao, Song Xiangwen, Chen Cunwu, Liu Dong, Han Bangxing, Yi Shanyong

机构信息

Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, P.R. China.

Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, West Anhui University, Lu'an, P.R. China.

出版信息

Mitochondrial DNA B Resour. 2022 Jun 20;7(6):1087-1089. doi: 10.1080/23802359.2022.2086496. eCollection 2022.

DOI:10.1080/23802359.2022.2086496
PMID:35756430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9225772/
Abstract

Diels, an important medicinal herb, is mainly distributed in Yunnan province, Southwest China. In this study, the complete chloroplast genome of was successfully sequenced. The assembled chloroplast genome was 155,108 bp in length with an overall GC content of 36.98%, including a pair of inverted repeat (IR) regions (26,573 bp, each), respectively, a large single-copy (LSC) region (84,848 bp) and a small single-copy (SSC) region (17,114 bp). The genome contained 131 genes, comprising 85 protein-coding genes, 37 tRNA genes, eight rRNA genes, and one pseudogene. The phylogenetic analysis indicated that was closely related to

摘要

地耳草是一种重要的药用植物,主要分布于中国西南部的云南省。在本研究中,成功测定了地耳草的完整叶绿体基因组序列。组装后的叶绿体基因组长度为155,108 bp,总体GC含量为36.98%,包括一对反向重复(IR)区域(各26,573 bp)、一个大单拷贝(LSC)区域(84,848 bp)和一个小单拷贝(SSC)区域(17,114 bp)。该基因组包含131个基因,包括85个蛋白质编码基因、37个tRNA基因、8个rRNA基因和1个假基因。系统发育分析表明,地耳草与……关系密切。

需注意,原文中“Diels”未明确具体所指植物,且最后“closely related to”后内容缺失,以上翻译为尽量符合语境的完整表述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c256/9225772/749cdf42c26d/TMDN_A_2086496_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c256/9225772/749cdf42c26d/TMDN_A_2086496_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c256/9225772/749cdf42c26d/TMDN_A_2086496_F0001_B.jpg

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本文引用的文献

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Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Diels Based on Transcriptome Data.基于转录组数据的 Diels 中定量 RT-PCR 分析合适参考基因的选择和验证。
Biomed Res Int. 2020 Jan 9;2020:5824841. doi: 10.1155/2020/5824841. eCollection 2020.
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Bioinformatics. 2018 Sep 1;34(17):i884-i890. doi: 10.1093/bioinformatics/bty560.
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GeSeq - versatile and accurate annotation of organelle genomes.
GeSeq - 细胞器基因组的多功能和准确注释。
Nucleic Acids Res. 2017 Jul 3;45(W1):W6-W11. doi: 10.1093/nar/gkx391.
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Biologically active arborinane-type triterpenoids and anthraquinones from Rubia yunnanensis.来自云南茜草的生物活性梓醇型三萜和蒽醌类化合物。
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PLoS One. 2010 Mar 10;5(3):e9490. doi: 10.1371/journal.pone.0009490.
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Identification of protein coding regions by database similarity search.通过数据库相似性搜索鉴定蛋白质编码区域。
Nat Genet. 1993 Mar;3(3):266-72. doi: 10.1038/ng0393-266.