口服给药诱导的小鼠肠道、脾脏和肝脏的炎症反应。

Inflammatory response of gut, spleen, and liver in mice induced by orally administered .

作者信息

Liu Yingman, Huang Wenkai, Dai Ke, Liu Ni, Wang Jiaqi, Lu Xiaoying, Ma Jiaojiao, Zhang Manman, Xu Mengqi, Long Xu, Liu Jie, Kou Yurong

机构信息

Department of Periodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning, China.

Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.

出版信息

J Oral Microbiol. 2022 Jun 16;14(1):2088936. doi: 10.1080/20002297.2022.2088936. eCollection 2022.

BACKGROUND

Periodontitis is a chronic multifactorial inflammatory disease. is a primary periopathogen in the initiation and development of periodontal disease. Evidence has shown that is associated with systemic diseases, including IBD and fatty liver disease. Inflammatory response is a key feature of diseases related to this species.

METHODS

C57BL/6 mice were administered either PBS, or . After 9 weeks, the inflammatory response in gut, spleen, and liver was analyzed.

RESULTS

The findings revealed significant disturbance of the intestinal microbiota and increased inflammatory factors in the gut of -administered mice. Administrated remarkably promoted the secretion of IRF-1 and activated the inflammatory pathway IFN-γ/STAT1 in the spleen. Histologically, mice treated with exhibited hepatocyte damage and lipid deposition. The inflammatory factors IL-17a, IL-6, and ROR-γt were also upregulated in the liver of mice fed with . Lee's index, spleen index, and liver index were also increased.

CONCLUSION

These results suggest that administrated evokes inflammation in gut, spleen, and liver, which might promote the progression of various systemic diseases.

背景

牙周炎是一种慢性多因素炎症性疾病。[某种细菌]是牙周疾病发生和发展的主要牙周病原体。证据表明，[该细菌]与包括炎症性肠病和脂肪性肝病在内的全身性疾病有关。炎症反应是与该物种相关疾病的一个关键特征。

方法

给C57BL/6小鼠注射磷酸盐缓冲盐水（PBS）或[某种细菌]。9周后，分析肠道、脾脏和肝脏中的炎症反应。

结果

研究结果显示，注射[某种细菌]的小鼠肠道微生物群出现明显紊乱，肠道内炎症因子增加。注射[某种细菌]显著促进了干扰素调节因子-1（IRF-1）的分泌，并激活了脾脏中的炎症途径γ干扰素/信号转导和转录激活因子1（IFN-γ/STAT1）。组织学上，用[某种细菌]处理的小鼠表现出肝细胞损伤和脂质沉积。喂食[某种细菌]的小鼠肝脏中炎症因子白细胞介素-17a（IL-17a）、白细胞介素-6（IL-6）和维甲酸相关孤儿受体γt（ROR-γt）也上调。李氏指数、脾脏指数和肝脏指数也增加。