Proteomics Research Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.
Department of Clinical Laboratory, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, China.
PeerJ. 2022 Jun 22;10:e13545. doi: 10.7717/peerj.13545. eCollection 2022.
Previous studies reported that sex and age could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. In this study, we provided a comprehensive lifespan characterization of urine metabolomics in a cohort of 348 healthy children and 315 adults, aged 1 to 78 years, using liquid chromatography coupled with high resolution mass spectrometry. Our results suggest that sex-dependent urine metabolites are much greater in adults than in children. The pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the geriatric stage. Based on the above analysis, metabolomic characteristics of each age stage were provided. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.
先前的研究报告指出，性别和年龄可能会影响尿液代谢组学，在生物标志物的发现中应予以考虑。因此，为了避免临床队列研究中的混杂效应，对于尿液代谢组学特征的基线，这一点变得至关重要。在这项研究中，我们使用液相色谱-高分辨质谱联用技术，对 348 名健康儿童和 315 名成人（年龄 1 至 78 岁）的尿液代谢组学进行了全面的生命周期特征描述。我们的结果表明，成年人体内依赖于性别的尿液代谢物比儿童体内多得多。泛酸和辅酶 A 生物合成以及丙氨酸代谢途径在生命早期就已被富集。雄激素和雌激素代谢在青少年和青年阶段表现出高活性。嘧啶代谢在老年期被富集。基于以上分析，提供了每个年龄阶段的代谢组学特征。这项工作有助于我们了解尿液代谢特征的基线，并有助于进一步研究临床疾病的生物标志物发现。
