Boorman J, Mellor P S, Penn M, Jennings M
Arch Virol. 1975;47(4):343-9. doi: 10.1007/BF01347975.
Seven-day-old embryonated hen eggs were infected with African Horse Sickness virus by the yolk sac and intravenous routes. Virus reached a high titre in the blood of infected embryos. Culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26 degrees - 27 degrees C transmitted African Horse Sickness virus to uninfected eggs. C. variipennis may therefore be considered a biological vector of African Horse Sickness virus in the laboratory.
将7日龄的鸡胚通过卵黄囊和静脉途径感染非洲马瘟病毒。病毒在受感染胚胎的血液中达到高滴度。从受感染的卵中吸食血液的库蠓感染了病毒，在26摄氏度至27摄氏度下放置7天后，将非洲马瘟病毒传播给未受感染的卵。因此，在实验室中，库蠓可被视为非洲马瘟病毒的生物传播媒介。
