The Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, NY, USA.
Department of Dermatology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Nanomedicine. 2022 Aug;44:102580. doi: 10.1016/j.nano.2022.102580. Epub 2022 Jun 26.
Ultraviolet Beam (UVB) radiation is the main cause of skin cancer worldwide. Besides biocompatibility, the instability and limited skin permeability are the most challenging features of many effective photochemopreventive agents. (-)-Epigallocatechin-3-gallate (EGCG) is a natural polyphenolic compound extracted from Camellia sinensis that has been demonstrated to have antioxidant, anti-inflammatory, and anti-cancer properties. We evaluated the efficacy of three innovative EGCG nanoformulations in chemoprevention of UVB-induced DNA damage in keratinocytes. Results indicated that the EGCG nanoformulations reduced UVB-induced oxidative stress elevation and DNA damage. The nanoformulations also reduced the UVB-induced formation of pyrimidine and pyrimidone photoproducts in 2D human immortalized HaCaT keratinocytes and SKH-1 hairless mice through antioxidant effects and possibly through absorption of UVB radiation. In addition, EGCG nanoformulations inhibited UVB-induced chemokine/cytokine activation and promoted EGCG skin permeability and stability. Taken together, the results suggest the use of EGCG nanoformulations as potential natural chemopreventive agents during exposure to UVB radiation.
紫外线（UVB）辐射是全球皮肤癌的主要原因。除了生物相容性外，许多有效光化学预防剂的不稳定性和有限的皮肤渗透性是最具挑战性的特征。（-）-表没食子儿茶素-3-没食子酸酯（EGCG）是一种从茶树中提取的天然多酚化合物，已被证明具有抗氧化、抗炎和抗癌特性。我们评估了三种创新的 EGCG 纳米制剂在预防角质细胞中 UVB 诱导的 DNA 损伤方面的功效。结果表明，EGCG 纳米制剂降低了 UVB 诱导的氧化应激升高和 DNA 损伤。纳米制剂还通过抗氧化作用，可能通过吸收 UVB 辐射，减少了 2D 人永生化 HaCaT 角质细胞和 SKH-1 无毛小鼠中嘧啶和嘧啶酮光产物的形成。此外，EGCG 纳米制剂抑制了 UVB 诱导的趋化因子/细胞因子激活，并促进了 EGCG 的皮肤渗透性和稳定性。总之，这些结果表明，在暴露于 UVB 辐射时，使用 EGCG 纳米制剂作为潜在的天然化学预防剂。
