Department of Biotechnology and Genetic Engineering, Hazara University, Mansehra, 21300, KP, Pakistan.
School of Biomedical Engineering, Dalian University of Technology, Dalian, China.
Microbiol Immunol. 2022 Sep;66(9):426-432. doi: 10.1111/1348-0421.13018. Epub 2022 Jul 18.
Dengue is a mosquito-borne viral illness that infects humans. For the past few decades, it has been declared a global public health problem. The current study, conducted at the district headquarter hospital (DHQ) Bannu between June to September 2018, was based on the seroprevalence of antibodies against dengue virus serotypes and their hematological parameters among the patients. A total of 1738 individuals suspected of having dengue were diagnosed through NS1, IgG, and IgM antibodies and RT-PCR techniques. Of all the samples, 716 (41.19%) were found to be positive for dengue. A higher infection rate was found in males (65.92%) compared with females (34.07%). The most affected age group was 16-40 years, whereas the most affected tehsil was Bannu, where the DENV-3 serotype was prevalent. The rare serotype (DENV-4) was found in 1% of cases. Symptoms including fever (100%), myalgia (100%), headache (61.31%), vomiting (34.63%), and rashes were common among the dengue patients. However, the mild cases showed fewer clinical signs compared with the severely infected cases. The study also revealed a significant association (P < 0.05) between hematological parameters and dengue infection, showing a significant decrease in TC, eosinophils, neutrophils, and platelets and a significant increase in monocytes and lymphocytes. Based on the current report, it is concluded that patients with the above symptoms and hematological changes may have an increased probability of dengue and should be kept under observation to separate dengue-positive patients and to enhance the treatment process.
登革热是一种由蚊子传播的病毒病，感染人类。在过去的几十年里，它已被宣布为全球公共卫生问题。本研究于 2018 年 6 月至 9 月在地区总部医院（DHQ）Bannu 进行，基于针对登革热病毒血清型的抗体血清阳性率及其在患者中的血液学参数。共有 1738 名疑似患有登革热的个体通过 NS1、IgG 和 IgM 抗体和 RT-PCR 技术进行诊断。在所有样本中，有 716 份（41.19%）被发现对登革热呈阳性。男性（65.92%）的感染率高于女性（34.07%）。受影响最大的年龄组是 16-40 岁，而受影响最大的 tehsil 是 Bannu，那里流行 DENV-3 血清型。罕见血清型（DENV-4）在 1%的病例中发现。发热（100%）、肌痛（100%）、头痛（61.31%）、呕吐（34.63%）和皮疹是登革热患者的常见症状。然而，与重度感染病例相比，轻度病例的临床症状较少。该研究还揭示了血液学参数与登革热感染之间存在显著关联（P<0.05），表明 TC、嗜酸性粒细胞、中性粒细胞和血小板显著减少，单核细胞和淋巴细胞显著增加。根据目前的报告，得出的结论是，出现上述症状和血液学变化的患者可能有更高的登革热概率，应进行观察以分离登革热阳性患者，并加强治疗过程。
