School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003, People's Republic of China.
Institute of Plant Protection, Key Lab of Food Quality and Safety of Jiangsu Province-State, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People's Republic of China.
Sci Rep. 2022 Jun 30;12(1):11081. doi: 10.1038/s41598-022-15188-5.
Fusarium oxysporum (Fo) is an important species complex of soil-borne pathogenic fungi that cause vascular wilt diseases of agricultural crops and some opportunistic diseases of humans. The fungicide phenamacril has been extensively reported to have antifungal activity against Fusarium graminearum and Fusarium fujikuroi. In this study, we found that the amino acid substitutions (V151A and S418T) in Type I myosin FoMyo5 cause natural low resistance to phenamacril in the plant pathogenic Fo isolates. Therefore, we compared the transcriptomes of two phenamacril-resistant Fo isolates FoII5, Fo1st and one phenamacril-sensitive isolate Fo3_a after 1 μg/mL phenamacril treatment. Among the 2728 differentially expressed genes (DEGs), 14 DEGs involved in oxidation-reduction processes and MFS transporters, were significantly up-regulated in phenamacril-resistant isolates. On the other hand, 14 DEGs involved in ATP-dependent RNA helicase and ribosomal biogenesis related proteins, showed significantly down-regulated expression in both phenamacril-resistant and -sensitive isolates. These results indicated that phenamacril not only seriously affected the cytoskeletal protein binding and ATPase activity of sensitive isolate, but also suppressed ribosome biogenesis in all the isolates. Hence, this study helps us better understand resistance regulation mechanism and fungicidal activity of phenamacril and provide reference for the development of new fungicides to control Fo.
尖孢镰刀菌（Fo）是一种重要的土传病原真菌物种复合体，可引起农业作物的维管束萎蔫病和一些人类机会性病。杀真菌剂苯霜灵已被广泛报道对禾谷镰刀菌和藤仓镰刀菌具有抗真菌活性。在本研究中，我们发现 I 型肌球蛋白 FoMyo5 中的氨基酸取代（V151A 和 S418T）导致植物病原 Fo 分离株对苯霜灵产生天然低抗性。因此，我们比较了在 1μg/mL 苯霜灵处理后，两种苯霜灵抗性 Fo 分离株 FoII5、Fo1st 和一种苯霜灵敏感分离株 Fo3_a 的转录组。在 2728 个差异表达基因（DEGs）中，14 个与氧化还原过程和 MFS 转运体相关的 DEGs 在苯霜灵抗性分离株中显著上调。另一方面，14 个与 ATP 依赖的 RNA 解旋酶和核糖体生物发生相关蛋白相关的 DEGs 在苯霜灵抗性和敏感分离株中均表现出显著下调表达。这些结果表明，苯霜灵不仅严重影响敏感分离株的细胞骨架蛋白结合和 ATP 酶活性，而且还抑制了所有分离株的核糖体生物发生。因此，本研究有助于我们更好地理解苯霜灵的抗性调控机制和杀菌活性，并为开发新的防治 Fo 的杀菌剂提供参考。
