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视网膜中的组胺。

Histamine in the retina.

作者信息

Ehinger B, Hallengren C

出版信息

Acta Physiol Scand. 1987 Feb;129(2):263-5. doi: 10.1111/j.1748-1716.1987.tb08067.x.

DOI:10.1111/j.1748-1716.1987.tb08067.x
PMID:3577813
Abstract

Retina and choroid were rapidly and gently extracted from chickens, guinea-pigs, rats, rabbits and cows. They were frozen, weighted and pooled in 3% trichloroacetic acid in a refrigerator. Using a radioenzymic assay, histamine was found to be present in the retina of guinea-pig (4.26 +/- 0.677 nmol g-1), rabbit (1.08 +/- 0.422 nmol g-1) and cow (0.189 +/- 0.036 nmol g-1), but not detected in chicken and rat. In the choroid, histamine was found in all species except rat (chicken: 4.92 +/- 0.542 nmol g-1; guinea-pig: 121.5 +/- 4.46 nmol g-1; rabbit: 18.7 +/- 3.93 nmol g-1; cow; 0.36 +/- 0.095 nmol g-1). The study verifies the presence of histamine in the retina of some species, and thereby supports previous fluorometry assays.

摘要

从鸡、豚鼠、大鼠、兔和牛身上快速轻柔地取出视网膜和脉络膜。将它们冷冻、称重后,置于冰箱中的3%三氯乙酸中混合。采用放射酶分析法,发现组胺存在于豚鼠(4.26±0.677纳摩尔/克)、兔(1.08±0.422纳摩尔/克)和牛(0.189±0.036纳摩尔/克)的视网膜中,但在鸡和大鼠中未检测到。在脉络膜中,除大鼠外,所有物种均发现有组胺(鸡:4.92±0.542纳摩尔/克;豚鼠:121.5±4.46纳摩尔/克;兔:18.7±3.93纳摩尔/克;牛:0.36±0.095纳摩尔/克)。该研究证实了某些物种视网膜中组胺的存在,从而支持了先前的荧光测定法。

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Histamine, ZO-1 and increased blood-retinal barrier permeability in diabetic retinopathy.组胺、紧密连接蛋白1与糖尿病视网膜病变中血视网膜屏障通透性增加
Trans Am Ophthalmol Soc. 1995;93:583-621.
2
Histamine-mediated regulation of cAMP levels and inositol phosphate metabolism in isolated rabbit retina.
Agents Actions. 1989 Apr;27(1-2):131-4. doi: 10.1007/BF02222219.
3
Histamine in the retina: recent progress and perspectives.
Agents Actions. 1990 Apr;30(1-2):202-5. doi: 10.1007/BF01969038.