Law Simon Sau Yin, Asanuma Masato, Shou Jingwen, Ozeki Yasuyuki, Kodama Yutaka, Numata Keiji
Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan.
Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
JACS Au. 2023 May 15;3(6):1604-1614. doi: 10.1021/jacsau.3c00041. eCollection 2023 Jun 26.
Plants can rapidly respond to different stresses by activating multiple signaling and defense pathways. The ability to directly visualize and quantify these pathways in real time using bioorthogonal probes would have practical applications, including characterizing plant responses to both abiotic and biotic stress. Fluorescence-based labels are widely used for tagging of small biomolecules but are relatively bulky and with potential effects on their endogenous localization and metabolism. This work describes the use of deuterium- and alkyne-derived fatty acid Raman probes to visualize and track the real-time response of plants to abiotic stress within the roots. Relative quantification of the respective signals could be used to track their localization and overall real-time responses in their fatty acid pools due to drought and heat stress without labor-intensive isolation procedures. Their overall usability and low toxicity suggest that Raman probes have great untapped potential in the field of plant bioengineering.
植物可以通过激活多种信号传导和防御途径快速响应不同的胁迫。使用生物正交探针实时直接可视化和量化这些途径的能力将具有实际应用价值，包括表征植物对非生物和生物胁迫的反应。基于荧光的标签广泛用于标记小分子生物分子，但相对较大且可能对其内源定位和代谢产生影响。这项工作描述了使用氘和炔烃衍生的脂肪酸拉曼探针来可视化和追踪植物根系对非生物胁迫的实时反应。由于干旱和热胁迫，无需费力的分离程序，各自信号的相对定量可用于追踪它们在脂肪酸库中的定位和整体实时反应。它们的整体可用性和低毒性表明拉曼探针在植物生物工程领域具有巨大的未开发潜力。
