Blee E, Durst F
Arch Biochem Biophys. 1987 Apr;254(1):43-52. doi: 10.1016/0003-9861(87)90079-8.
The sulfoxidation of methiocarb, an aromatic-alkyl sulfide pesticide, catalyzed by soybean microsomes was found to be strongly stimulated in the presence of cumene and linoleic acid hydroperoxides. We have shown that this S-oxidation, which does not require cofactors such as NAD(P)H, is an hydroperoxide-dependent reaction: 18O2-labeling experiments demonstrated that the oxygen atom incorporated into the sulfoxide originated from hydroperoxides rather than from molecular oxygen. In the absence of exogenous hydroperoxides, soybean microsomes catalyzed methiocarb sulfoxide formation at a basal rate dependent on their endogenous hydroperoxides, especially those derived from free fatty acids. The nature of the sulfoxidase is discussed. Our results seem to rule out the participation of cytochrome P-450 in this oxidation, whereas the studied sulfoxidase presents some similarities to plant peroxygenase.
研究发现，在异丙苯和亚油酸氢过氧化物存在的情况下，大豆微粒体催化的芳香族烷基硫醚农药甲硫威的亚砜氧化反应受到强烈刺激。我们已经表明，这种不需要NAD(P)H等辅因子的S-氧化反应是一种依赖氢过氧化物的反应：18O2标记实验表明，掺入亚砜中的氧原子来自氢过氧化物而非分子氧。在没有外源氢过氧化物的情况下，大豆微粒体以依赖其内源性氢过氧化物（尤其是那些源自游离脂肪酸的氢过氧化物）的基础速率催化甲硫威亚砜的形成。本文讨论了亚砜氧化酶的性质。我们的结果似乎排除了细胞色素P-450参与这种氧化反应的可能性，而所研究的亚砜氧化酶与植物过氧合酶有一些相似之处。
