Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Microb Pathog. 2022 Aug;169:105667. doi: 10.1016/j.micpath.2022.105667. Epub 2022 Jul 3.
The Pathology of digestive tract has long been known to be correlated with chronic kidney disease (CKD). The member of the major Firmicutes phylum especially Clostridium subcluster XIVa altered quantitatively and qualitatively in the gut microbiota of patients with End Stage Renal Disease (ESRD) and CKD. Therefore, in this study, the abundance of the species of Clostridium genus of Firmicutes phylum compared between intestine microbiota of patients with kidney failure and healthy individual. Fresh fecal specimens of 20 patients at different stages of CKD and 20 healthy individuals were collected. Bacterial DNA of samples were extracted to use for 16S ribosomal DNA sequencing targeting the V3-V4 region. Next generation sequencing (NGS) method at MiSeq system was used to find the diversity of gut microbiota composition. Totally, 11 (1.68%) of 651 bacterial strains which were isolated from forty fecal samples of both healthy volunteers and CKD/ESRD patients, were identified as Clostridium species. Eight genera of 11 Clostridium genera were related to Clostridium sensu stricto, and 3 other genera were as follows Vallitalea, Acidaminobacter and Caloramator. Among both group, the highest abundance was dedicated to Clostridium celatum genera. Sarcina maxima were not identified. The composition of Clostridium spp. showed the same frequency among CKD/ESRD and healthy groups (p < 0.05). The abundance of Clostridium spp. is virtually the same and not differs among healthy individuals and CKD/ESRD patients. Results of the present indicate despite of critical role of gut microbiota, some pathogens and their metabolites have no role on hemostasis and pathogenesis of kidney disorders.
消化道的病理学长期以来一直被认为与慢性肾脏病（CKD）有关。厚壁菌门的主要梭状芽胞杆菌亚群成员在终末期肾病（ESRD）和 CKD 患者的肠道微生物群中在数量和质量上都发生了改变。因此，在这项研究中，将比较肾衰竭和健康个体肠道微生物群中厚壁菌门梭状芽胞杆菌属的物种丰度。收集了 20 名不同 CKD 阶段的患者和 20 名健康个体的新鲜粪便标本。提取样本的细菌 DNA，用于靶向 V3-V4 区的 16S 核糖体 DNA 测序。使用 MiSeq 系统的下一代测序（NGS）方法来发现肠道微生物群落组成的多样性。从 40 份健康志愿者和 CKD/ESRD 患者的粪便样本中总共分离出 651 株细菌（1.68%），鉴定为梭菌属。11 株梭菌属中的 8 个属与严格梭菌属有关，另外 3 个属分别为 Vallitalea、Acidaminobacter 和 Caloramator。在这两个组中，梭状芽胞杆菌属的丰度最高。未鉴定出巨大芽胞杆菌。梭菌属的组成在 CKD/ESRD 和健康组之间显示出相同的频率（p < 0.05）。健康个体和 CKD/ESRD 患者之间梭菌属的丰度几乎相同，没有差异。本研究结果表明，尽管肠道微生物群具有重要作用，但一些病原体及其代谢物与肾脏疾病的止血和发病机制无关。
