Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Arch Razi Inst. 2021 Sep 1;76(3):521-528. doi: 10.22092/ari.2020.352271.1557. eCollection 2021 Summer.
family are gut microbiota that exhibit probiotic or health promoting effects on the host. Several studies have suggested that gut microbiota are quantitatively and qualitatively altered in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). The present study aimed to assess the members of family in fecal samples of patients with CKD and ESRD and compare them with non-CKD/ESRD patients to find any changes in their counts and diversions in these patients. Twenty fresh fecal samples from patients with CKD/ESRD and twenty from non-CKD/ESRD patients were examined. Whole DNA was extracted from fecal samples and the gut microbiota composition was analyzed by next generation sequencing (NGS). A total of 651 strains were identified from 40 fecal samples, 8 (1.23%) strains of which were identified as family . The most abundant species in both control and disease groups were and subsp. , and the least abundant species in the disease group was subsp. lactis. There was no significant difference in the abundance of various species between the disease and control groups (p < 0.05). This study confirms that the members of the family are not altered in patients with CKD/ESRD.
家族是肠道微生物群，对宿主表现出益生菌或促进健康的作用。几项研究表明，慢性肾脏病（CKD）和终末期肾病（ESRD）患者的肠道微生物群在数量和质量上都发生了改变。本研究旨在评估 CKD 和 ESRD 患者粪便样本中家族成员的情况，并将其与非 CKD/ESRD 患者进行比较，以发现这些患者的计数变化和多样性变化。检查了 20 例 CKD/ESRD 患者和 20 例非 CKD/ESRD 患者的新鲜粪便样本。从粪便样本中提取全 DNA，并通过下一代测序（NGS）分析肠道微生物群组成。从 40 个粪便样本中鉴定出 651 株，其中 8 株（1.23%）鉴定为家族。在对照组和疾病组中最丰富的物种是和亚种，而疾病组中最少的物种是乳亚种。疾病组和对照组之间各种物种的丰度没有显著差异（p < 0.05）。本研究证实，CKD/ESRD 患者的家族成员没有改变。
