开发和验证用于非人类灵长类动物监测的 SARS-CoV-2 检测方法。
Developing and validating SARS-CoV-2 assays for nonhuman primate surveillance.
机构信息
Primate Assay Laboratory, California National Primate Research Center, University of California, Davis, Davis, California, USA.
Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
出版信息
J Med Primatol. 2022 Oct;51(5):264-269. doi: 10.1111/jmp.12604. Epub 2022 Jul 6.
INTRODUCTION
In early 2020, the California National Primate Research Center implemented surveillance to address the threat of SARS-CoV-2 infection in its nonhuman primate colony.
MATERIALS/METHODS: To detect antiviral antibodies, multi-antigen assays were developed and validated on enzyme immunoassay and multiplex microbead immunofluorescent assay (MMIA) platforms. To detect viral RNA, RT-PCR was also performed.
RESULTS/CONCLUSION: Using a 4plex, antibody was identified in 16/16 experimentally infected animals; and specificity for spike, nucleocapsid, receptor binding domain, and whole virus antigens was 95.2%, 93.8%, 94.3%, and 97.1%, respectively on surveillance samples. Six laboratories compared this MMIA favorably with nine additional laboratory-developed or commercially available assays. Using a screen and confirm algorithm, 141 of the last 2441 surveillance samples were screen-reactive requiring confirmatory testing. Although 35 samples were reactive to either nucleocapsid or spike; none were reactive to both. Over 20 000 animals have been tested and no spontaneous infections have so far been confirmed across the NIH sponsored National Primate Research Centers.
简介
2020 年初，加利福尼亚国家灵长类动物研究中心实施了监测，以应对其非人类灵长类动物群体中 SARS-CoV-2 感染的威胁。
材料/方法：为了检测抗病毒抗体，开发并验证了多抗原酶免疫分析和多重微球免疫荧光分析（MMIA）平台上的检测方法。为了检测病毒 RNA，还进行了 RT-PCR。
结果/结论：使用 4 重检测方法，在 16/16 只实验感染的动物中发现了抗体；在监测样本中，针对刺突蛋白、核衣壳蛋白、受体结合域和全病毒抗原的特异性分别为 95.2%、93.8%、94.3%和 97.1%。六个实验室将这种 MMIA 与其他九个实验室开发或商业可用的检测方法进行了比较，结果非常好。使用筛选和确认算法，在最后 2441 个监测样本中的 141 个呈筛检反应，需要进行确认性检测。尽管 35 个样本对核衣壳蛋白或刺突蛋白呈反应性；但没有一个同时对两者都呈反应性。在 NIH 赞助的国家灵长类动物研究中心，已经对超过 20000 只动物进行了检测，到目前为止，还没有确认过自发性感染。
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