Wang Xiaohu, Ren Zhaowen, Wang Lu, Chen Jing, Zhang Pian, Chen Jin-Ping, Chen Xiaofan, Li Linmiao, Lin Xuhui, Qi Nanshan, Luo Shengjun, Xiang Rong, Yuan Ziguo, Zhang Jianfeng, Wang Gang, Sun Min-Hua, Huang Yuan, Hua Yan, Zou Jiejian, Hou Fanghui, Huang Zhong, Du Shouwen, Xiang Hua, Sun Mingfei, Liu Quan, Liao Ming
Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Baishigang, Wushan Street, Tianhe District, Guangzhou 510640, China.
Institute of Zoology, Guangdong Academy of Sciences, No. 105 Xingang West Road, Haizhu District, Guangzhou 510260, China.
Virus Evol. 2022 Jun 21;8(1):veac049. doi: 10.1093/ve/veac049. eCollection 2022.
Coronavirus infections cause diseases that range from mild to severe in mammals and birds. In this study, we detected coronavirus infections in 748 farmed wild animals of 23 species in Guangdong, southern China, by RT-PCR and metagenomic analysis. We identified four coronaviruses in these wild animals and analysed their evolutionary origins. Coronaviruses detected in were genetically grouped into canine and rodent coronaviruses, which were likely recombinants of canine and rodent coronaviruses. The coronavirus found in was a recombinant pheasant coronavirus of turkey coronavirus and infectious bronchitis virus. The coronavirus in had a high nucleotide identity (94.6-98.5 per cent) with a coronavirus of bottlenose dolphin (). These findings suggested that the wildlife coronaviruses may have experienced homologous recombination and/or crossed the species barrier, likely resulting in the emergence of new coronaviruses. It is necessary to reduce human-animal interactions by prohibiting the eating and raising of wild animals, which may contribute to preventing the emergence of the next coronavirus pandemic.
冠状病毒感染在哺乳动物和鸟类中会引发从轻度到重度不等的疾病。在本研究中，我们通过逆转录聚合酶链反应（RT-PCR）和宏基因组分析，在中国南方广东省对23个物种的748只养殖野生动物进行了冠状病毒感染检测。我们在这些野生动物中鉴定出四种冠状病毒，并分析了它们的进化起源。在[具体动物1]中检测到的冠状病毒在基因上被归类为犬冠状病毒和啮齿动物冠状病毒，它们可能是犬冠状病毒和啮齿动物冠状病毒的重组体。在[具体动物2]中发现的冠状病毒是火鸡冠状病毒和传染性支气管炎病毒的重组野鸡冠状病毒。在[具体动物3]中的冠状病毒与宽吻海豚的一种冠状病毒具有较高的核苷酸同一性（94.6%-98.5%）。这些发现表明，野生动物冠状病毒可能经历了同源重组和/或跨越了物种屏障，这可能导致了新的冠状病毒的出现。有必要通过禁止食用和养殖野生动物来减少人与动物的接触，这可能有助于预防下一次冠状病毒大流行的出现。
