Greenlee J E, Dodd W K
Arch Virol. 1987;94(1-2):169-73. doi: 10.1007/BF01313736.
Murine K-papovavirus was serially passaged 10 times in primary cultures of mouse embryo cells, using cell suspensions and media from infected cultures to inoculate fresh flasks at each passage level. Titers of K virus infectivity in cell suspensions and media rose during serial passage, but viral hemagglutinating activity was not detected in cells or media before the 10th passage. Although the infectivity of K virus for mouse embryo cultures was enhanced by serial passage, lethality of the virus for suckling mice was lost. The present study represents the first successful serial transmission of K virus infection in vitro.
鼠K乳头多瘤病毒在小鼠胚胎细胞原代培养物中连续传代10次，每次传代时使用来自感染培养物的细胞悬液和培养基接种新的培养瓶。在连续传代过程中，细胞悬液和培养基中K病毒的感染性滴度升高，但在第10次传代之前，在细胞或培养基中未检测到病毒血凝活性。虽然连续传代增强了K病毒对小鼠胚胎培养物的感染性，但该病毒对乳鼠的致死性丧失。本研究代表了K病毒感染在体外首次成功的连续传播。
