Wroblewska Z, Wellish M, Gilden D
Arch Virol. 1980;65(2):141-8. doi: 10.1007/BF01317325.
Adult human brain (AHB) cells infected with JC virus (JCV) developed a cytopathic effect (CPE) beginning 12--14 days after infection. Ultrastructurally, 37--40 nm papova virions were seen in the nuclei of infected cells, and both T and V antigen were demonstrated by indirect immunofluorescence. The hemagglutinating titer of JCV in infected AHB cells was 10--40 times higher than the amount of JCV used to initiate infection. AHB cells are more readily available than primary human fetal brain cells, they can be subcultured 15--25 times in vitro and they support JCV replication after multiple subcultivations. These properties make the AHB cell line useful for propagating JCV.
感染JC病毒（JCV）的成人人类脑细胞（AHB）在感染后12 - 14天开始出现细胞病变效应（CPE）。在超微结构上，在感染细胞的细胞核中可见37 - 40纳米的乳头多瘤空泡病毒颗粒，并且通过间接免疫荧光法证实了T抗原和V抗原。感染AHB细胞中的JCV血凝滴度比用于引发感染的JCV量高10 - 40倍。AHB细胞比原代人类胎儿脑细胞更容易获得，它们可以在体外传代培养15 - 25次，并且在多次传代培养后仍支持JCV复制。这些特性使得AHB细胞系可用于JCV的繁殖。
