Ottoboni L M, Steffensen D M
Biochem Genet. 1987 Feb;25(1-2):123-42. doi: 10.1007/BF00498956.
The band patterns of zein polypeptides were determined for many commercial inbred corn lines and maize stocks using isofocusing in agarose gels and sodium dodecyl sulfate (SDS)-urea gels. Each inbred line or homozygous maize strain genotype has a distinct zein profile which has been catalogued according to the distance of charge migration and molecular weight (kilodaltons). Several zein polypeptides were mapped to chromosomes 4 and 10 with the use of reciprocal translocations. The mapping of at least two polypeptides on distal 4L and 10L had not been previously reported. The general methods used in the present research will permit the mapping of all the zein polypeptides to chromosomal sites.
利用琼脂糖凝胶等聚焦和十二烷基硫酸钠（SDS）-尿素凝胶，测定了许多商业近交玉米系和玉米种质的醇溶蛋白多肽的条带模式。每个近交系或纯合玉米品系基因型都有独特的醇溶蛋白谱，已根据电荷迁移距离和分子量（千道尔顿）进行了编目。利用相互易位将几种醇溶蛋白多肽定位到了第4和第10号染色体上。此前尚未报道至少两种多肽在4L和10L远端的定位情况。本研究中使用的通用方法将能够把所有醇溶蛋白多肽定位到染色体位点。
