Wilson C M
Biochem Genet. 1985 Feb;23(1-2):115-24. doi: 10.1007/BF00499117.
Isoelectric focusing of zein in agarose gels gives sharp separations of at least 25 bands noted among 25 corn-belt inbreds. Six inbreds provided standard bands which were used to construct a pattern map. A method is provided for comparing bands, identified by distance from the cathode, which differ only slightly in position. The 25 inbreds were separated into five groups on the basis of pattern similarity. Some groups contained inbreds derived from widely different sources. Zein isoelectric focusing in agarose should be useful for genotype identification and for determination of varietal purity.
玉米醇溶蛋白在琼脂糖凝胶中的等电聚焦能清晰分离出25个玉米带自交系中的至少25条带。六个自交系提供了标准带，用于构建模式图谱。提供了一种比较仅在位置上略有不同的带的方法，这些带通过距阴极的距离来识别。根据模式相似性，将这25个自交系分为五组。有些组包含来源差异很大的自交系。琼脂糖中的玉米醇溶蛋白等电聚焦应有助于基因型鉴定和品种纯度测定。
