Jakovljević Violeta D, Radojević Ivana D, Grujić Sandra M, Ostojić Aleksandar M
Department for Science and Mathematics, State University of Novi Pazar, Vuka Karadžića 9, 36300 Novi Pazar, Serbia.
Institute for Biology and Ecology, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
Saudi J Biol Sci. 2022 Aug;29(8):103347. doi: 10.1016/j.sjbs.2022.103347. Epub 2022 Jun 16.
The goal of the current study was to examine the effects of pollutants (White color - CP; Metallic red color - FM; Thinner - CN; Thinner for rinsing paint - MF; Basic color (primer) - FH) originating from the automotive industry on the biofilm growth, matrix protein content, and activity of the hydrolytic enzymes of selected microbial strains in laboratory conditions that mimic the bioreactor conditions. The chosen microorganisms (bacteria, yeasts, and fungi) were isolated from automotive industry wastewater. Pure microbe cultures and their consortia were injected into AMB Media carriers and developed into biofilms. The use of AMB media carriers has been linked to an increase in the active surface area colonized by microorganisms. Afterwards, the carriers were transferred to Erlenmeyer flasks with nutrient media and pollutants at a concentration of 200 μL/mL. The current study found that, depending on the microbial strain, development phase, and chemical structure, the assessed pollutants had an inhibitory or stimulatory influence on the growth of single cultures and their consortia. Statistical analysis found positive correlations between the protein content in the matrix and the biofilm biomass of and consortia in CP and FH media, respectively. The proteolytic activity of was very pronounced in media with MF and CN. The best alkaline phosphatase activity (ALP) was achieved in the CN medium of . Acid invertase activity was the highest in the FM and CP media of and consortia, respectively, whereas the highest alkaline invertase activity was measured in the MF medium of A positive correlation was confirmed between ALP and the biofilm biomass of in CP and CN media, as well as between ALP and the biofilm biomass of in FM medium. The findings provide novel insights into the extracellular hydrolytic activity of the investigated microbial strains in the presence of auto paints, as well as a good platform for subsequent research into comprehensive biofilm profiling using modern methodologies.
本研究的目的是在模拟生物反应器条件的实验室环境中，考察源自汽车行业的污染物（白色颜料 - CP；金属红色颜料 - FM；稀释剂 - CN；洗漆稀释剂 - MF；基础色（底漆） - FH）对所选微生物菌株生物膜生长、基质蛋白含量及水解酶活性的影响。所选微生物（细菌、酵母和真菌）从汽车行业废水中分离得到。将纯微生物培养物及其混合菌群接种到AMB培养基载体上，使其形成生物膜。使用AMB培养基载体与微生物定殖的活性表面积增加有关。之后，将载体转移至含有浓度为200 μL/mL营养培养基和污染物的锥形瓶中。本研究发现，根据微生物菌株、发育阶段和化学结构，所评估的污染物对单一培养物及其混合菌群的生长具有抑制或刺激作用。统计分析分别发现CP和FH培养基中基质蛋白含量与单一培养物及混合菌群生物膜生物量之间存在正相关。在含有MF和CN的培养基中，单一培养物的蛋白水解活性非常显著。在单一培养物的CN培养基中，碱性磷酸酶活性（ALP）最高。在单一培养物及混合菌群的FM和CP培养基中，酸性转化酶活性分别最高，而在单一培养物的MF培养基中，碱性转化酶活性测得最高。在CP和CN培养基中，ALP与单一培养物的生物膜生物量之间，以及在FM培养基中，ALP与混合菌群的生物膜生物量之间均证实存在正相关。这些发现为研究汽车涂料存在下所调查微生物菌株的细胞外水解活性提供了新的见解，同时也为后续使用现代方法进行综合生物膜分析的研究提供了良好的平台。
