Guo Yuan, Gao Qi, Fan Yangyang, Song Shuang, Yan Dong, Zhao Jing, Chen Yulin, Liu Yu, Wang Shouxian
Beijing Engineering Research Center for Edible Mushroom, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China.
J Fungi (Basel). 2023 Jan 29;9(2):179. doi: 10.3390/jof9020179.
Temperature type is one of the key traits determining the cultivation regime of . However, the molecular and metabolic basis underling temperature type remain unclear. Here, we investigated the phenotypic, transcriptomic, and metabolic features of with different temperature types under both control (25 °C) and high (37 °C) temperature conditions. We found that under the control condition, the high- and low-temperature types of harbored distinct transcriptional and metabolic profiles. The high-temperature (H-)-type strain had a higher expression level of genes involved in the toxin processes and carbohydrate binding, while the low-temperature (L-)-type strain had a high expression level of oxidoreductase activity. Heat stress significantly inhibited the growth of both H- and L-type strains, while the latter had a higher growth inhibition rate. Upon exposure to heat, the H-type strain significantly up-regulated genes associated with the components of the cellular membrane, whereas the L-type strain markedly up-regulated genes involved in the extracellular region and carbohydrate binding. Metabolome data showed that thermostress altered purine and pyrimidine metabolism in the H-type strain, whereas it altered cysteine, methionine, and glycerophospholipid metabolism in the L-type strain. Transcriptome and metabolome integrative analysis was able to identify three independent thermotolerance-related gene-metabolite regulatory networks. Our results deepen the current understanding of the molecular and metabolic basis underlying temperature type and suggest, for the first time, that thermotolerance mechanisms can be temperature-type-dependent for .
温度类型是决定[具体研究对象]培养方式的关键特征之一。然而，温度类型背后的分子和代谢基础仍不清楚。在此，我们研究了在对照（25°C）和高温（37°C）条件下不同温度类型的[具体研究对象]的表型、转录组和代谢特征。我们发现，在对照条件下，[具体研究对象]的高温型和低温型具有不同的转录和代谢谱。高温（H-）型菌株参与毒素过程和碳水化合物结合的基因表达水平较高，而低温（L-）型菌株氧化还原酶活性的表达水平较高。热应激显著抑制了H型和L型菌株的生长，而后者的生长抑制率更高。受热后，H型菌株显著上调了与细胞膜成分相关的基因，而L型菌株则显著上调了参与细胞外区域和碳水化合物结合的基因。代谢组数据表明，热应激改变了H型菌株中的嘌呤和嘧啶代谢，而改变了L型菌株中的半胱氨酸、甲硫氨酸和甘油磷脂代谢。转录组和代谢组综合分析能够识别出三个独立的耐热性相关基因-代谢物调控网络。我们的结果加深了目前对温度类型背后分子和代谢基础的理解，并首次表明耐热机制可能因[具体研究对象]的温度类型而异。
