Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Particles-Biology Interactions, 9014, St. Gallen, Switzerland.
Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, 9014, St. Gallen, Switzerland.
Sci Rep. 2022 Jul 8;12(1):11583. doi: 10.1038/s41598-022-15471-5.
The COVID-19 pandemic has caused considerable interest worldwide in antiviral surfaces, and there has been a dramatic increase in the research and development of innovative material systems to reduce virus transmission in the past few years. The International Organization for Standardization (ISO) norms 18,184 and 21,702 are two standard methods to characterize the antiviral properties of porous and non-porous surfaces. However, during the last years of the pandemic, a need for faster and inexpensive characterization of antiviral material was identified. Therefore, a complementary method based on an Inactivated Virus System (InViS) was developed to facilitate the early-stage development of antiviral technologies and quality surveillance of the production of antiviral materials safely and efficiently. The InViS is loaded with a self-quenched fluorescent dye that produces a measurable increase in fluorescence when the viral envelope disintegrates. In the present work, the sensitivity of InViS to viral disintegration by known antiviral agents is demonstrated and its potential to characterize novel materials and surfaces is explored. Finally, the InViS is used to determine the fate of viral particles within facemasks layers, rendering it an interesting tool to support the development of antiviral surface systems for technical and medical applications.
新冠疫情在全球范围内引发了对抗病毒表面的极大兴趣，在过去几年中，人们大力研究和开发创新材料系统，以减少病毒传播。国际标准化组织（ISO）标准 18,184 和 21,702 是两种用于表征多孔和非多孔表面抗病毒性能的标准方法。然而，在疫情的最后几年，人们需要一种更快、更经济的抗病毒材料特性描述方法。因此，开发了一种基于灭活病毒系统（InViS）的补充方法，以促进抗病毒技术的早期开发，并安全有效地对抗病毒材料的生产进行质量监测。InViS 加载了一种自猝灭荧光染料，当病毒包膜破裂时，会产生可测量的荧光增强。在本工作中，证明了 InViS 对已知抗病毒剂引起的病毒解体的敏感性，并探讨了其表征新型材料和表面的潜力。最后，将 InViS 用于确定口罩层内病毒颗粒的命运，使其成为支持开发用于技术和医疗应用的抗病毒表面系统的有趣工具。
