Zhang Wen, Yu Ying, He Huihui, Lv Xucong, Liu Zhibin, Ni Li
Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China.
Foods. 2022 Jun 27;11(13):1913. doi: 10.3390/foods11131913.
is a typical spoilage bacteria organism in seafood. The adhesion ability of three strains (HR-15, JR-18, HC-71) isolated from putrefied tilapia were evaluated by mucus adhesion in vitro and intestinal adhesion in vivo. The results of the spoilage of the inoculated fish fillets and spoilage of the refrigerated fish both showed that the adhesion ability of was positively correlated with the spoilage ability. High-throughput sequencing and GC-MS results showed that with high adhesion ability also significantly changed the intestinal flora of fish, causing an increase in the intestinal bacteria such as , and , which then led to the increase in volatile substances such as low-grade aldehydes, alcohols, and ketones in the fish, serious fatty acid oxidation, and excitement of the fishy smell.
是海鲜中一种典型的腐败细菌有机体。通过体外黏液黏附试验和体内肠道黏附试验,对从腐败罗非鱼中分离出的三株菌(HR - 15、JR - 18、HC - 71)的黏附能力进行了评估。接种鱼片的腐败结果和冷藏鱼的腐败结果均表明,其黏附能力与腐败能力呈正相关。高通量测序和气相色谱 - 质谱联用结果表明,黏附能力强的菌也显著改变了鱼的肠道菌群,导致诸如 、 和 等肠道细菌增加,进而导致鱼体内低级醛、醇和酮等挥发性物质增加,脂肪酸氧化严重,腥味加剧。