Dong Li-Feng, Xu Shu-Ying, Long Jing-Pei, Wan Fang, Chen Yi-Ding
Department of Breast, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China (mainland).
Physical Examination Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China (mainland).
Med Sci Monit. 2017 Jun 11;23:2842-2849. doi: 10.12659/msm.904789.
BACKGROUND Breast cancer is one of the most common malignancies in women. In a previous study, we found that for two patients who had a high risk of lymphatic metastasis, lymphatic metastasis did not occur; whereas, for two patients who had a low risk of lymphatic metastasis, lymphatic metastasis did occur. MATERIAL AND METHODS We analyzed the differential gene expressions of these four patients by RNA-sequence. The data (HRNM_T versus HRNM_N, LRYM_T versus LRYM_N, and HRNM_T versus LRYM_T) was then processed using differentially expressed genes (DEGs) analysis, functional analysis for DEGs, and PPI network construct. RESULTS For HRNM_T versus HRNM_N, there were 224 DEGs. There were 504 DEGs for LRYM_T versus LRYM_N, and 88 DEGs for LRYM_T versus LRYM_N. For HRNM_T versus HRNM_N, DEGs were up-regulated mainly in the cell cycle, the IL-17 signaling pathway, and the progesterone-mediated oocyte maturation; DEGs were down-regulated mainly in the IL-17 signaling pathway. For LRYM_T versus LRYM_N, DEGs were up-regulated mainly in protein digestion and absorption, and cytokine-cytokine receptor interaction; DEGs were down-regulated mainly in ECM-receptor interaction. For HRNM_T versus LRYM_T, DEGs were up-regulated mainly in the PPAR signaling pathway; DEGs were downregulated mainly in the adipocytokine signaling pathway. The DEGs were screened to construct PPI networks. CONCLUSIONS The GO and KEGG functional enrichments of HRNM_T versus HRNM_N, and LRYM_T versus LRYM_N were consistent with earlier studies. For HRNM_T versus LRYM_T, DEGs were up-regulated mainly in PPAR signaling; DEGs were down-regulated mainly in the adipocytokine pathway.
乳腺癌是女性最常见的恶性肿瘤之一。在先前的一项研究中，我们发现，对于两名有高淋巴转移风险的患者，并未发生淋巴转移；然而，对于另外两名低淋巴转移风险的患者，却发生了淋巴转移。
我们通过RNA测序分析了这四名患者的差异基因表达。然后使用差异表达基因（DEG）分析、DEG功能分析和PPI网络构建对数据（HRNM_T与HRNM_N、LRYM_T与LRYM_N以及HRNM_T与LRYM_T）进行处理。
对于HRNM_T与HRNM_N，有224个差异表达基因。LRYM_T与LRYM_N有504个差异表达基因，HRNM_T与LRYM_T有88个差异表达基因。对于HRNM_T与HRNM_N，差异表达基因主要在细胞周期、IL-17信号通路和孕酮介导的卵母细胞成熟中上调；差异表达基因主要在IL-17信号通路中下调。对于LRYM_T与LRYM_N，差异表达基因主要在蛋白质消化和吸收以及细胞因子-细胞因子受体相互作用中上调；差异表达基因主要在细胞外基质-受体相互作用中下调。对于HRNM_T与LRYM_T，差异表达基因主要在PPAR信号通路中上调；差异表达基因主要在脂肪细胞因子信号通路中下调。对差异表达基因进行筛选以构建PPI网络。
HRNM_T与HRNM_N以及LRYM_T与LRYM_N的GO和KEGG功能富集与早期研究一致。对于HRNM_T与LRYM_T，差异表达基因主要在PPAR信号中上调；差异表达基因主要在脂肪细胞因子通路中下调。
