Kim Yejin, Kim Jaeyoon, Son So-Ri, Kim Ji-Young, Choi Jung-Hye, Jang Dae Sik
Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Korea.
Plants (Basel). 2022 Jun 22;11(13):1651. doi: 10.3390/plants11131651.
The flower of (Puerariae Flos) is a reddish-purple to violet-purple flower that blooms between July and September. In our preliminary study, Puerariae Flos extract exhibited significant activity against a human ovarian cancer cell line. This research aims to identify the active compounds in Pueraria Flos. By repeated chromatography, one new tryptophan derivative (), two new flavanones ( and ), and 19 known compounds, including tryptophan derivatives ( and ), flavonoids (-), isoflavonoids (-), a flavonolignan (), and a phenolic compound (), were isolated from a methanol extract of Puerariae Flos. The structures of new compounds were elucidated as 13--benzoyl-l-tryptophan-1--d-glucopyranoside (), 2-hydroxy-5-methoxy-naringenin (), and 2-hydroxy-5-methoxy-naringenin 7---d-glucopyranoside (). Among the isolates, afromosin (), tectorigenin (), apigenin (), glycitein (), (-)-hydnocarpin (), irilin D (), irisolidone 7--glucoside (), and genistein () showed cytotoxicity against human ovarian cancer cell line A2780. Apigenin () and (-)-hydnocarpin () were the most active (IC values of 9.99 and 7.36 μM, respectively).
葛花是一种红紫色至紫堇色的花，在7月至9月间开放。在我们的初步研究中，葛花提取物对人卵巢癌细胞系表现出显著活性。本研究旨在鉴定葛花中的活性成分。通过反复柱色谱法，从葛花甲醇提取物中分离得到1个新的色氨酸衍生物（）、2个新的黄烷酮（和）以及19个已知化合物，包括色氨酸衍生物（和）、黄酮类化合物（-）、异黄酮类化合物（-）、一个黄酮木脂素（）和一个酚类化合物（）。新化合物的结构被鉴定为13--苯甲酰基-l-色氨酸-1--d-吡喃葡萄糖苷（）、2-羟基-5-甲氧基柚皮素（）和2-羟基-5-甲氧基柚皮素7---d-吡喃葡萄糖苷（）。在所分离的化合物中，阿弗罗莫辛（）、鸢尾黄素（）、芹菜素（）、大豆黄素（）、（-）-副大风子油酸（）、艾瑞林D（）、鸢尾酮苷7--葡萄糖苷（）和染料木黄酮（）对人卵巢癌细胞系A2780表现出细胞毒性。芹菜素（）和（-）-副大风子油酸（）活性最强（IC值分别为9.99和7.36 μM）。
