Tasinov Oskan, Dincheva Ivayla, Badjakov Ilian, Grupcheva Christina, Galunska Bistra
Department of Biochemistry, Molecular Medicine and Nutrigenomics, Faculty of Pharmacy, Medical University of Varna, 84B Tzar Osvoboditel Blvd., 9002 Varna, Bulgaria.
AgroBioInstitute, Agricultural Academy, 8 Dr. Tsankov Blvd., 1164 Sofia, Bulgaria.
Plants (Basel). 2022 Jun 22;11(13):1655. doi: 10.3390/plants11131655.
L. (black chokeberry), belonging to the Rosaceae family, contains high amounts of polyphenolics and therefore exhibits one of the highest antioxidant and anti-inflammatory activities among berry fruits. Chokeberries are used in the food industry for juice, nectar, and wine production and as colorants. We aimed to compare the phytochemical composition of three chokeberry juices commercially available in the local market as sources of beneficial phytochemicals. Using GC-MS and LC-MS/MS, we performed the identification and quantitation of polar compounds and polyphenolics. The concentrations of 13 amino acids, including 6 essential amino acids, 10 organic acids, 20 sugar alcohols and derivatives, 14 saccharides, 12 fatty acids and esters, and 38 polyphenols, were estimated. One of the analyzed juices had the highest polyphenolic content (5273.87 ± 63.16 µg/mL), possibly due to 2.9 times higher anthocyanin concentration compared to anthocyanins in other tested juices. This study provides new data concerning phytochemical composition in terms of amino acids, organic acids, sugar acids, fatty acids and their esters, and polyphenols as phytocomponents of commercially available chokeberry juices. Results show that after all processing techniques and possibly different plant growth conditions, chokeberry juices are a valuable source of health-promoting phytochemicals such as phenolic acids, pro-anthocyanins, and anthocyanins, thus considering them as functional foods. We demonstrated a diversity of the active substances in bioactive foods marketed as "same"; therefore, the standardized therapeutic effect could be expected only by the utilization of food supplements with guaranteed constant content.
黑果腺肋花楸（L.，黑 chokeberry）属于蔷薇科，含有大量多酚类物质，因此在浆果类水果中具有极高的抗氧化和抗炎活性。黑果腺肋花楸在食品工业中用于生产果汁、花蜜和葡萄酒，并用作色素。我们旨在比较当地市场上三种市售黑果腺肋花楸果汁的植物化学成分，这些果汁是有益植物化学物质的来源。我们使用气相色谱 - 质谱联用仪（GC-MS）和液相色谱 - 串联质谱仪（LC-MS/MS）对极性化合物和多酚类物质进行了鉴定和定量。估计了13种氨基酸（包括6种必需氨基酸）、10种有机酸、20种糖醇及其衍生物、14种糖类、12种脂肪酸和酯类以及38种多酚的浓度。其中一种分析的果汁具有最高的多酚含量（5273.87±63.16μg/mL），这可能是由于其花青素浓度比其他测试果汁中的花青素高2.9倍。本研究提供了关于市售黑果腺肋花楸果汁中氨基酸、有机酸、糖酸、脂肪酸及其酯类以及多酚等植物成分的植物化学成分的新数据。结果表明，经过所有加工技术以及可能不同的植物生长条件后，黑果腺肋花楸果汁是促进健康的植物化学物质（如酚酸、原花青素和花青素）的宝贵来源，因此可将其视为功能性食品。我们证明了作为“相同”销售的生物活性食品中活性物质的多样性；因此，只有通过使用保证含量恒定的食品补充剂才能预期获得标准化的治疗效果。
