Shaffique Shifa, Khan Muhammad Aaqil, Wani Shabir Hussain, Imran Muhammad, Kang Sang-Mo, Pande Anjali, Adhikari Arjun, Kwon Eun-Hae, Lee In-Jung
Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea.
Center of Biotechnology and Microbiology, University of Peshawar, Peshawar 45000, Pakistan.
Plants (Basel). 2022 Jun 24;11(13):1674. doi: 10.3390/plants11131674.
Maize is the third most common cereal crop worldwide, after rice and wheat, and plays a vital role in preventing global hunger crises. Approximately 50% of global crop yields are reduced by drought stress. Bacteria as biostimulants for biopriming can improve yield and enhance sustainable food production. Further, seed biopriming stimulates plant defense mechanisms. In this study, we isolated bacteria from the rhizosphere of plants from Pohang beach, Daegu, South Korea. Twenty-three isolates were isolated and screened for growth promoting potential. Among them, bacterial isolate SH-6 was selected based on maximum induced tolerance to polyethylene glycol-simulated drought. SH-6 showed ABA concentration = 1.06 ± 0.04 ng/mL, phosphate solubilizing index = 3.7, and sucrose concentration = 0.51 ± 0.13 mg/mL. The novel isolate SH-6 markedly enhanced maize seedling tolerance to oxidative stress owing to the presence of superoxide dismutase, catalase, and ascorbate peroxidase activities in the culture media. Additionally, we quantified and standardized the biopriming effect of SH-6 on maize seeds. SH-6 significantly increased maize seedling drought tolerance by up to 20%, resulting in 80% germination potential. We concluded that the novel bacterium isolate SH-6 (gene accession number (OM757882) is a biostimulant that can improve germination performance under drought stress.
玉米是全球第三大常见谷类作物，仅次于水稻和小麦，在预防全球饥饿危机方面发挥着至关重要的作用。全球约50%的作物产量因干旱胁迫而减产。细菌作为生物引发的生物刺激剂可以提高产量并促进可持续粮食生产。此外，种子生物引发还能刺激植物防御机制。在本研究中，我们从韩国大邱浦项海滩植物的根际分离出细菌。分离出23株菌株并筛选其促生长潜力。其中，基于对聚乙二醇模拟干旱的最大诱导耐受性，选择了细菌分离株SH-6。SH-6的脱落酸浓度 = 1.06 ± 0.04 ng/mL，解磷指数 = 3.7，蔗糖浓度 = 0.51 ± 0.13 mg/mL。由于培养基中存在超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶活性，新分离株SH-6显著增强了玉米幼苗对氧化应激的耐受性。此外，我们对SH-6对玉米种子的生物引发效果进行了量化和标准化。SH-6使玉米幼苗的耐旱性显著提高了20%，发芽潜力达到80%。我们得出结论，新细菌分离株SH-6（基因登录号(OM757882)）是一种生物刺激剂，可在干旱胁迫下提高发芽性能。
