Fu Manman, Wu Chao, Li Xia, Ding Xiaoyu, Guo Fangqi
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310058, China.
Plants (Basel). 2022 Jul 1;11(13):1760. doi: 10.3390/plants11131760.
Calmodulin (CaM) and calmodulin-like proteins (CML) act as significant Ca sensors binding Ca with EF-hand motifs and have been reported to be involved in various environmental stresses in plants. In this study, calmodulin gene family members were identified based on the genome of published recently; a phylogenetic tree was constructed; gene structures and chromosomal locations of were depicted; cis-acting regulatory elements were predicted; collinearity and duplicate events of were analyzed using MCScanX software; and the expression levels of in response to abiotic stress were analyzed, based on the published RNA-seq data. We identified 86 (4 and 82 ) genes in total. Promoter sequences of contained elements related to abiotic stresses (including low-temperature and anaerobic stresses) and plant hormones (including abscisic acid (ABA), MeJA, and salicylic acid). genes were distributed on nine chromosomes unevenly. Collinearity analysis indicated that recent segmental duplications significantly enlarged the scale of the family in . Four (, , , and ) were statistically differentially regulated under low-temperature and salt stress compared with those in the normal condition. These results indicate diverse roles of in plant development and in response to environmental stimuli in .
钙调蛋白（CaM）和类钙调蛋白（CML）作为重要的钙传感器，通过EF-手基序结合钙离子，据报道它们参与植物的各种环境胁迫。在本研究中，基于最近公布的基因组鉴定了钙调蛋白基因家族成员；构建了系统发育树；描绘了基因结构和染色体位置；预测了顺式作用调控元件；使用MCScanX软件分析了共线性和重复事件；并基于已发表的RNA-seq数据，分析了其对非生物胁迫的响应表达水平。我们总共鉴定出86个钙调蛋白基因（4个CaM和82个CML）。钙调蛋白基因的启动子序列包含与非生物胁迫（包括低温和厌氧胁迫）和植物激素（包括脱落酸（ABA）、茉莉酸甲酯（MeJA）和水杨酸）相关的元件。钙调蛋白基因不均匀地分布在9条染色体上。共线性分析表明，近期的片段重复显著扩大了钙调蛋白基因家族在该植物中的规模。与正常条件下相比，4个钙调蛋白基因（CaM1、CaM2、CML3和CML4）在低温和盐胁迫下受到统计学上的差异调控。这些结果表明钙调蛋白基因在该植物的发育和对环境刺激的响应中具有多种作用。
