Rodríguez Miguel, Reina José Carlos, Sampedro Inmaculada, Llamas Inmaculada, Martínez-Checa Fernando
Department of Microbiology, Faculty of Pharmacy, University of Granada, Granada, Spain.
Biomedical Research Centre (CIBM), Institute of Biotechnology, University of Granada, Granada, Spain.
Front Plant Sci. 2022 Jun 23;13:896728. doi: 10.3389/fpls.2022.896728. eCollection 2022.
A strictly aerobic, chemoheterotrophic, endospore-forming, Gram-positive, rod-shaped bacterial strain N3 was isolated from the feces of a river otter in Castril (Granada, southern Spain). It is halotolerant, motile, and catalase-, oxidase-, ACC deaminase-, and C4- and C8-lipase-positive. It promotes tomato plant growth and can reduce virulence in CECT 222 and LMG 25993 through interference in their quorum-sensing systems, although other antagonistic mechanisms could also occur. A phylogenetic analysis of the 16S rRNA gene sequence as well as the phenotypic and phylogenomic analyses indicated that the strain N3 is a novel species of the genus , with the highest 16S rRNA sequence similar to that of DSM 8801 (99.93%) and DSM 1321 (99.80%). Genomic digital DNA-DNA hybridization (dDDH) between the strain N3 and DSM 8801 and was 12.8 and 69.1%, respectively, and the average nucleotide identity (ANIb) of strain N3 and DSM 8801 and was 67.84 and 93.21%, respectively. The genomic G + C content was 40.3 mol%. Its main cellular fatty acids were anteiso-C and iso-C. Using 16S rRNA phylogenetic and phylogenomic analyses, together with the chemotaxonomic and phenotypic data, we demonstrated that the type strain N3 (=CECT 30509 = LMG 32505) is a novel species of the genus and the name sp. nov. is proposed.
从西班牙南部格拉纳达省卡斯蒂尔的一只水獭粪便中分离出一株严格需氧、化能异养、产芽孢、革兰氏阳性、杆状的细菌菌株N3。它耐盐、具运动性，过氧化氢酶、氧化酶、ACC脱氨酶以及C4和C8脂肪酶呈阳性。它能促进番茄植株生长，并且可以通过干扰群体感应系统降低CECT 222和LMG 25993的毒力，不过也可能存在其他拮抗机制。对16S rRNA基因序列进行的系统发育分析以及表型和系统基因组分析表明，菌株N3是该属的一个新物种，其16S rRNA序列与DSM 8801（99.93%）和DSM 1321（99.80%）最为相似。菌株N3与DSM 8801以及DSM 1321之间的基因组数字DNA-DNA杂交（dDDH）分别为12.8%和69.1%，菌株N3与DSM 8801以及DSM 1321的平均核苷酸同一性（ANIb）分别为67.84%和93.21%。基因组G + C含量为40.3 mol%。其主要细胞脂肪酸为anteiso-C和iso-C。通过16S rRNA系统发育和系统基因组分析，结合化学分类和表型数据，我们证明模式菌株N3（=CECT 30509 = LMG 32505）是该属的一个新物种，并提出新物种名为sp. nov.。
