Lin Yi-Xin, Xu Hai-Jin, Yin Guang-Kun, Zhou Yuan-Chang, Lu Xin-Xiong, Xin Xia
National Crop Genebank, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Agriculture, Fujian Agricultural and Forestry University, Fuzhou, China.
Front Plant Sci. 2022 Jun 24;13:908949. doi: 10.3389/fpls.2022.908949. eCollection 2022.
Seed viability depends upon the maintenance of functional lipids; however, how membrane lipid components dynamically change during the seed aging process remains obscure. Seed storage is accompanied by the oxidation of membrane lipids and loss of seed viability. Understanding membrane lipid changes and their effect on the cell membrane during seed aging can contribute to revealing the mechanism of seed longevity. In this study, the potential relationship between oxidative stress and membrane lipid metabolism was evaluated by using a non-targeted lipidomics approach during artificial aging of L. Merr. Zhongdou No. 27 seeds. We determined changes in reactive oxygen species, malondialdehyde content, and membrane permeability and assessed antioxidant system activity. We found that decreased non-enzymatic antioxidant contents and catalase activity might lead to reactive oxygen species accumulation, resulting in higher electrolyte leakage and lipid peroxidation. The significantly decreased phospholipids and increased glycerolipids and lysophospholipids suggested that hydrolysis of phospholipids to form glycerolipids and lysophospholipids could be the primary pathway of membrane metabolism during seed aging. Moreover, the ratio of phosphatidylcholine to phosphatidylethanolamine, double bond index, and acyl chain length of phospholipids were found to jointly regulate membrane function. In addition, the observed changes in lipid metabolism suggest novel potential hallmarks of soybean seed aging, such as diacylglycerol 36:4; phosphatidylcholine 34:2, 36:2, and 36:4; and phosphatidylethanolamine 34:2. This knowledge can be of great significance for elucidating the molecular mechanism underlying seed aging and germplasm conservation.
种子活力取决于功能性脂质的维持；然而，在种子老化过程中膜脂成分如何动态变化仍不清楚。种子储存伴随着膜脂的氧化和种子活力的丧失。了解种子老化过程中膜脂的变化及其对细胞膜的影响有助于揭示种子寿命的机制。在本研究中，通过非靶向脂质组学方法评估了人工老化的大豆中豆27种子氧化应激与膜脂代谢之间的潜在关系。我们测定了活性氧、丙二醛含量和膜通透性的变化，并评估了抗氧化系统活性。我们发现非酶抗氧化剂含量和过氧化氢酶活性的降低可能导致活性氧积累，从而导致更高的电解质渗漏和脂质过氧化。磷脂显著减少，甘油olipids和溶血磷脂增加，这表明磷脂水解形成甘油olipids和溶血磷脂可能是种子老化过程中膜代谢的主要途径。此外，还发现磷脂酰胆碱与磷脂酰乙醇胺的比例、双键指数和磷脂的酰基链长度共同调节膜功能。此外，观察到的脂质代谢变化表明大豆种子老化有新的潜在标志，如二酰基甘油36:4；磷脂酰胆碱34:2、36:2和36:4；以及磷脂酰乙醇胺34:2。这些知识对于阐明种子老化和种质保存的分子机制具有重要意义。
