Liu Zhixiao, Tong Hua, Li Jian, Wang Ling, Fan Xiaoyi, Song Honghao, Yang Mei, Wang Haowei, Jiang Xin, Zhou Xuhui, Yuan Hongbin, Wang Yue
Department of Histology and Embryology, College of Basic Medicine, Shanghai, China.
Department of Anesthesiology, Changzheng Hospital, Shanghai, China.
Front Bioeng Biotechnol. 2022 Jun 23;10:922570. doi: 10.3389/fbioe.2022.922570. eCollection 2022.
A hydrogel system loaded with mesenchymal stem cell-derived exosome (MSC-Exos) is an attractive new tool for tissue regeneration. However, the effect of the stiffness of exosome-loaded hydrogels on tissue regeneration is unclear. Here, the role of exosome-loaded hydrogel stiffness, during the regeneration of injured nerves, was assessed . The results showed that the photocrosslinkable hyaluronic acid methacrylate hydrogel stiffness plays an important role in repairing nerve injury. Compared with the stiff hydrogels loaded with exosomes, soft hydrogels loaded with exosomes showed better repair of injured peripheral nerves. The soft hydrogel promoted nerve repair by quickly releasing exosomes to inhibit the infiltration of macrophages and the expression of the proinflammatory factors IL-1β and TNF-α in injured nerves. Our work revealed that exosome-loaded hydrogel stiffness plays an important role in tissue regeneration by regulating exosome release behavior and provided important clues for the clinical application of biological scaffold materials.
负载间充质干细胞衍生外泌体（MSC-Exos）的水凝胶系统是一种用于组织再生的新型有吸引力的工具。然而，负载外泌体的水凝胶的硬度对组织再生的影响尚不清楚。在此，评估了负载外泌体的水凝胶硬度在损伤神经再生过程中的作用。结果表明，光可交联的甲基丙烯酸透明质酸水凝胶硬度在修复神经损伤中起重要作用。与负载外泌体的硬水凝胶相比，负载外泌体的软水凝胶对损伤的周围神经显示出更好的修复效果。软水凝胶通过快速释放外泌体来抑制巨噬细胞浸润以及损伤神经中促炎因子IL-1β和TNF-α的表达，从而促进神经修复。我们的工作揭示了负载外泌体的水凝胶硬度通过调节外泌体释放行为在组织再生中起重要作用，并为生物支架材料的临床应用提供了重要线索。
