Zhu Jie, Liu Zhixiao, Wang Ling, Jin Qishu, Zhao Yunpeng, Du Antong, Ding Neng, Wang Yue, Jiang Hua, Zhu Lie
Department of Plastic and Reconstructive Surgery, Second Affiliated Hospital of Naval Medical University, Shanghai, China.
Department of Histology and Embryology, College of Basic Medicine, Naval Medical University, Shanghai, China.
Front Bioeng Biotechnol. 2022 May 20;10:866505. doi: 10.3389/fbioe.2022.866505. eCollection 2022.
Loading human umbilical mesenchymal stem cell (hUMSC) derived exosomes onto hydrogel scaffolds is a strategy for rapid wound healing. The clinical application of exosomes is hindered by low production, and exosome mimetics could be substituted for exosomes. Here, the therapeutic effects of exosome-loaded hydrogels and exosome mimetic-loaded hydrogels on wounds are evaluated. Our results revealed that exosome mimetic-loaded hydrogels promote wound healing more efficiently than exosome-loaded hydrogels. Exosome mimetics can promote the proliferation and migration of dermal fibroblasts (hDF-a) cells . To investigate how exosome mimetics play a role, proteomics analysis was applied, and the obtained results suggested that exosome mimetics significantly enrich mitochondrial-derived oxidative phosphorylation-related proteins in comparison to exosomes. Overall, our work envisages the emerging potential of exosome mimetics, which take the advantage of exosomes and can be promising candidates for exosomes. It also suggests that hUMSC-derived exosome mimetic-loaded hydrogels have remarkable prospects for clinical application.
将人脐间充质干细胞（hUMSC）衍生的外泌体负载到水凝胶支架上是一种促进伤口快速愈合的策略。外泌体的临床应用受到产量低的阻碍，外泌体模拟物可替代外泌体。在此，评估了负载外泌体的水凝胶和负载外泌体模拟物的水凝胶对伤口的治疗效果。我们的结果显示，负载外泌体模拟物的水凝胶比负载外泌体的水凝胶更有效地促进伤口愈合。外泌体模拟物可促进真皮成纤维细胞（hDF-a）的增殖和迁移。为了研究外泌体模拟物如何发挥作用，进行了蛋白质组学分析，所得结果表明，与外泌体相比，外泌体模拟物显著富集了线粒体衍生的氧化磷酸化相关蛋白。总体而言，我们的工作设想了外泌体模拟物的新兴潜力，其利用了外泌体的优势，有望成为外泌体的替代物。这也表明，负载hUMSC衍生外泌体模拟物的水凝胶具有显著的临床应用前景。
