Jongebloed W L, Humalda D, van Andel P, Worst J F
Doc Ophthalmol. 1986 Dec 30;64(1):129-42. doi: 10.1007/BF00166694.
The substructure of both the epithelial and endothelial surfaces of a keratoconus and an artificially aged cornea was compared with that of a healthy cornea by investigating them with a scanning electron microscope. From the depressions around the protruding centre of the epithelial surface of the keratoconus cornea, and from the whole epithelial surface of the artificially aged cornea, cells detached themselves, assuming a more or less rounded shape. The endothelial surface of both the keratoconus and the aged cornea showed areas of cells with an almost completely disintegrated cell membrane, exposing the cell contents. On the endothelial surface of the keratoconus cells were found with a missing cell-nucleus and a perforated cell membrane, due to a 'Kammerwasser Einbruch' effect.
通过扫描电子显微镜对圆锥角膜和人工老化角膜的上皮和内皮表面的亚结构与健康角膜进行了比较。在圆锥角膜上皮表面突出中心周围的凹陷处，以及人工老化角膜的整个上皮表面，细胞自行脱离，呈现出或多或少的圆形。圆锥角膜和老化角膜的内皮表面均显示出细胞膜几乎完全解体的细胞区域，暴露出细胞内容物。在圆锥角膜的内皮表面发现一些细胞核缺失且细胞膜穿孔的细胞，这是由于“房水侵入”效应所致。
