NHC Key Laboratory of Birth Defects Prevention, Henan Key Laboratory of Population Defects Prevention, Henan Institute of Reproduction Health Science and Technology, Zhengzhou, 450002, Henan Province, China.
BMC Pharmacol Toxicol. 2022 Jul 12;23(1):47. doi: 10.1186/s40360-022-00593-3.
Resveratrol (RSV) is a polyphenol compound found in grapes, veratrum and other plants. It has been reported that RSV has anti-inflammatory, anti-oxidant, anti-cancer and other pharmacological effects. However, the impacts of RSV on development of nervous system are not understood well. The study aims to investigate RSV's neuroprotective effect during development and to provide a health care for pregnant women and their fetuses with RSV supplementation.
In this study, we induced human induced pluripotent stem cells (hiPSCs) to form the embryoid bodies (EBs) and cerebral organoids (COs) with 3 dimensional (3D) culture. In the meantime, D-galactose (D-gal, 5 mg/ml) was used to make nervous injury model, and on the other hand, RSV with various doses, such as 2 μm/L, 10 μm/L, 50 μm/L, were applied to understand its neuroprotection. Therefore, the cultures were divided into control group, D-gal nervous injury group and RSV intervention groups. After that, the diameters of EBs and COs were measured regularly under a reverted microscope. In the meantime, the neural proliferation, cell apoptosis and the differentiation of germ layers were detected via immunofluorescence.
(1) D-gal could delay the development of EBs and COs; (2) RSV could rescue the atrophy of EBs and COs caused by D-gal; (3) RSV showed its neuroprotection, through promoting the neural cell proliferation, inhibiting apoptosis and accelerating the differentiation of germ layers.
RSV has a neuroprotective effect on the development of the nervous system, suggesting RSV supplementation may be necessary during the health care of pregnancy and childhood.
白藜芦醇（RSV）是一种存在于葡萄、藜芦和其他植物中的多酚化合物。已有报道称 RSV 具有抗炎、抗氧化、抗癌等药理作用。然而，RSV 对神经系统发育的影响尚不清楚。本研究旨在探讨 RSV 在发育过程中的神经保护作用，并为孕妇及其胎儿补充 RSV 提供保健作用。
在本研究中，我们诱导人诱导多能干细胞（hiPSCs）形成具有三维（3D）培养的类胚体（EBs）和类脑器官（COs）。同时，使用半乳糖（D-gal，5mg/ml）制作神经损伤模型，另一方面，用不同剂量的 RSV（如 2μm/L、10μm/L、50μm/L）进行干预，以了解其神经保护作用。因此，将培养物分为对照组、D-gal 神经损伤组和 RSV 干预组。之后，在倒置显微镜下定期测量 EBs 和 COs 的直径。同时，通过免疫荧光检测神经细胞增殖、细胞凋亡和胚层分化。
（1）D-gal 可延缓 EBs 和 COs 的发育；（2）RSV 可挽救 D-gal 引起的 EBs 和 COs 萎缩；（3）RSV 通过促进神经细胞增殖、抑制细胞凋亡和加速胚层分化表现出神经保护作用。
RSV 对神经系统的发育具有神经保护作用，提示在妊娠和儿童保健期间补充 RSV 可能是必要的。
