Noryan Morteza, Hervan Islam Majidi, Sabouri Hossein, Kojouri Faroukh Darvish, Mastinu Andrea
Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran 1477893855, Iran.
Department of Plant Production, Collage of Agricultural Science and Natural Resources, Gonbad Kavous University, Gonbad Kavous 4971799151, Iran.
BioTech (Basel). 2021 Nov 6;10(4):26. doi: 10.3390/biotech10040026.
In order to locate control genes related to L. traits at the germination stage under normal conditions and at drought stress levels (-4.5 and -9.0 bar), we evaluated 120 F8 generation offspring from the cross between two cultivars Neda × Ahlemitarum in a factorial experiment in a completely randomized block design with three replications in 2013 in the botanical laboratory of Gonbad Kavous University. A linkage map was prepared using 90 Simple Sequence Repeats (SSR) markers and 28 Inter Simple Sequence Repeats (ISSR), and 6 iPBS and 9 IRAP markers (265 polymorphic alleles). The results of the analysis of variance showed that all of the evaluated traits had a significant difference at the probability level of 1%. Hence, it can be noted that the desired genetic diversity can be found between genotypes. The results of the stepwise regression analysis for the germination percentage as a dependent variable and other traits as independent variables in the studied treatments showed that under normal conditions, there was variable coleoptile length, but under drought stress of -4.5 and -9.0 bar, the variable plumule dry weight entered the model. In this study, the markers included in RM1-RM490 and ISSR2-3-RM133 of chromosomes 1 and 6 of were identified as the main regulators of traits associated with drought resistance. In particular, they present the quantitative trait loci (QTL) that control the first stages of germination of in water stress conditions.
为了定位在正常条件下以及干旱胁迫水平（-4.5和-9.0巴）发芽阶段与L.性状相关的控制基因，2013年我们在贡巴德卡武斯大学植物实验室采用完全随机区组设计、三重复的析因试验，对两个品种Neda×Ahlemitarum杂交产生的120个F8代后代进行了评估。利用90个简单序列重复（SSR）标记、28个简单序列重复区间（ISSR）标记、6个iPBS标记和9个IRAP标记（265个多态性等位基因）构建了连锁图谱。方差分析结果表明，所有评估性状在1%的概率水平上均存在显著差异。因此，可以注意到在基因型之间能够找到所需的遗传多样性。以发芽率为因变量、其他性状为自变量在研究处理中进行逐步回归分析的结果表明，在正常条件下，胚芽鞘长度存在变异，但在-4.5和-9.0巴的干旱胁迫下，胚干重进入了模型。在本研究中，位于第1和第6号染色体的RM1-RM490以及ISSR2-3-RM133中的标记被确定为与抗旱性相关性状的主要调控因子。特别是，它们呈现了在水分胁迫条件下控制L.发芽初期的数量性状位点（QTL）。
