Mohun T, Garrett N, Treisman R
EMBO J. 1987 Mar;6(3):667-73. doi: 10.1002/j.1460-2075.1987.tb04806.x.
Xenopus laevis cytoskeletal actin gene promoters contain a 20-bp sequence homologous to the serum response element (SRE) required for transient human c-fos gene transcription in response to serum factors. Both sequences bind the same factor in HeLa cell extracts, as shown by binding competition, DNase I and dimethylsulphate (DMS) protection and DMS interference assays. A similar protein is present in Xenopus laevis oocytes. Sequences containing the SRE homology are essential for constitutive activity of the actin promoter in both Xenopus and mouse cells, and a synthetic SRE functions as a promoter element in these cells. In mouse cells, transcription of both transfected Xenopus actin and actin/c-fos fusion genes is activated following serum stimulation. These data suggest that the SRE and its cognate protein form part of a regulatory pathway that has been highly conserved during evolution.
非洲爪蟾细胞骨架肌动蛋白基因启动子包含一段20个碱基对的序列，该序列与血清反应元件（SRE）同源，血清反应元件是人类c-fos基因在对血清因子作出反应时进行瞬时转录所必需的。结合竞争、DNA酶I和硫酸二甲酯（DMS）保护以及DMS干扰试验表明，这两个序列在HeLa细胞提取物中结合相同的因子。非洲爪蟾卵母细胞中存在一种类似的蛋白质。含有SRE同源性的序列对于肌动蛋白启动子在非洲爪蟾和小鼠细胞中的组成型活性至关重要，并且一个合成的SRE在这些细胞中作为启动子元件发挥作用。在小鼠细胞中，血清刺激后，转染的非洲爪蟾肌动蛋白基因和肌动蛋白/c-fos融合基因的转录均被激活。这些数据表明，SRE及其同源蛋白构成了一条在进化过程中高度保守的调控途径的一部分。
