Kumar Sarita, Sharma Aarti, Samal Roopa Rani, Kumar Manoj, Verma Vaishali, Sagar Ravinder Kumar, Singh ShriPati, Raghavendra Kamaraju
Department of Zoology, Acharya Narendra Dev College, University of Delhi, Kalkaji, New Delhi 110 019, India.
National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi 110 077, India.
J Trop Med. 2022 Jun 18;2022:2977454. doi: 10.1155/2022/2977454. eCollection 2022.
Attractive toxic sugar bait (ATSB), based on "attract and kill" approach, is a novel and promising strategy for mosquito control. Formulation of an attractive sugar bait (ASB) solution by selecting an efficient olfaction stimulant and preparation of an optimized sugar-attractant dosage is a significant component for the success of the approach.
Current study evaluated relative potential of nine ASBs, formulated by combination of sugar and fresh fruit juices (guava, mango, muskmelon, orange, papaya, pineapple, plum, sweet lemon, and watermelon) in attracting adults. Freshly extracted and 48-hour-fermented juices were combined with 10% sucrose solution (w/v) in 1 : 1 ratio. Cage bioassays were conducted against two laboratory strains (susceptible: AND-; deltamethrin-selected: AND--DL10) and two field-collected strains (Shahdara strain of : SHD-Delhi; Govindpuri strain of : GVD-Delhi). Each of the nine ASBs was assayed, individually or in groups of three, for its attraction potential based on the relative number of mosquito landings. The data were analysed for statistical significance using PASW (SPSS) software 19.0 program.
The prescreening bioassay with individual ASB revealed significantly higher efficacy of ASB containing guava/plum/mango juice than that containing six other juices ( < 0.05) against both the laboratory and field strains. The bioassay with three ASBs kept in one cage, one of the effective ASBs and two others randomly selected ASBs, also showed good attractancy of the guava/plum/mango juice-ASB ( < 0.05). The postscreening assays with these three ASBs revealed maximum attractant potential of guava juice-sucrose combination for all the four strains of . Guava juice-ASB showed the highest attractancy against both laboratory and field-collected strains and can be used to formulate ATSB by combining with a toxicant. The field studies with these formulations will ascertain their efficacy and possible use in mosquito management programs.
基于“引诱并杀灭”方法的诱蚊毒性糖饵（ATSB）是一种新颖且有前景的蚊虫控制策略。通过选择高效嗅觉刺激物来配制诱蚊糖饵（ASB）溶液，并制备优化的糖引诱剂剂量，是该方法成功的重要组成部分。
本研究评估了由糖与新鲜果汁（番石榴、芒果、甜瓜、橙子、木瓜、菠萝、李子、甜柠檬和西瓜）组合配制的9种ASB对成蚊的相对引诱潜力。将新鲜提取的和发酵48小时的果汁与10%蔗糖溶液（w/v）按1∶1比例混合。针对两种实验室品系（敏感品系：AND-；溴氰菊酯选育品系：AND--DL10）和两种野外采集品系（德里沙达拉品系：SHD-德里；德里戈文德普里品系：GVD-德里）进行笼内生物测定。基于蚊子着陆的相对数量，对9种ASB中的每一种单独或按三种一组进行引诱潜力测定。使用PASW（SPSS）软件19.0程序对数据进行统计学显著性分析。
对单个ASB进行的预筛选生物测定显示，含番石榴/李子/芒果汁的ASB对实验室品系和野外品系的效力均显著高于含其他六种果汁的ASB（P<0.05）。对置于一个笼子中的三种ASB（一种有效ASB和另外两种随机选择的ASB）进行的生物测定也显示，含番石榴/李子/芒果汁的ASB具有良好的引诱性（P<0.05）。对这三种ASB进行的筛选后测定显示，番石榴汁 - 蔗糖组合对所有四种品系的引诱潜力最大。番石榴汁 - ASB对实验室品系和野外采集品系均表现出最高的引诱性，可通过与杀虫剂组合用于配制ATSB。对这些制剂进行的野外研究将确定它们在蚊虫管理计划中的效力和可能用途。
