通过蛋白质组学分析探究HO胁迫下KM1的抗氧化机制

Antioxidant Mechanism of KM1 Under HO Stress by Proteomics Analysis.

作者信息

Tian Yuan, Wang Yu, Zhang Nan, Xiao Minmin, Zhang Jing, Xing Xinyue, Zhang Yue, Fan Yuling, Li Xia, Nan Bo, Wang Yuhua, Liu Jingsheng

机构信息

College of Food Science, Jilin Agricultural University, Changchun, China.

Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China.

出版信息

Front Microbiol. 2022 Jun 27;13:897387. doi: 10.3389/fmicb.2022.897387. eCollection 2022.

KM1 was screened from natural fermented products, which had probiotic properties and antioxidant function. The survival rate of KM1 was 78.26% at 5 mM HO. In this study, the antioxidant mechanism of KM1 was deeply analyzed by using the proteomics method. The results demonstrated that a total of 112 differentially expressed proteins (DEPs) were screened, of which, 31 DEPs were upregulated and 81 were downregulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that DEPs participated in various metabolic pathways such as pyruvate metabolism, carbon metabolism, trichloroacetic acid cycle, amino acid metabolism, and microbial metabolism in diverse environments. These metabolic pathways were related to oxidative stress caused by HO in KM1. Therefore, the antioxidant mechanism of KM1 under HO stress provided a theoretical basis for its use as a potential natural antioxidant.

KM1是从天然发酵产物中筛选出来的，具有益生菌特性和抗氧化功能。在5 mM过氧化氢（HO）条件下，KM1的存活率为78.26%。在本研究中，采用蛋白质组学方法深入分析了KM1的抗氧化机制。结果表明，共筛选出112个差异表达蛋白（DEP），其中31个DEP上调，81个DEP下调。京都基因与基因组百科全书（KEGG）富集分析表明，DEP参与了多种代谢途径，如丙酮酸代谢、碳代谢、三羧酸循环、氨基酸代谢以及不同环境中的微生物代谢。这些代谢途径与KM1中由HO引起的氧化应激有关。因此，KM1在HO胁迫下的抗氧化机制为其作为潜在天然抗氧化剂的应用提供了理论依据。