Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Technology of Beijing, Ministry of Agriculture, Beijing, China.
J Appl Microbiol. 2022 Mar;132(3):2306-2322. doi: 10.1111/jam.15341. Epub 2021 Nov 17.
To evaluation the probiotic potential of Lactobacillus plantarum strain RW1 isolated from healthy dogs for its further utilization as a dietary supplement for dogs.
This study aimed to evaluate the probiotic potential of L. plantarum strain RW1 isolated from canine faeces. After confirming by conventional and then by 16S rRNA sequencing, the identified strain RW1 was in vitro screened for its survivability in simulated gastrointestinal conditions, low pH, bile salts and adhesion to gut epithelial tissues, growth inhibitory effects on common pathogens and anti-inflammatory potential by measuring the mRNA expression level of IL-6, IL-8, IL-1β in Salmonella-infected MODE-K cells. Furthermore, the effects on epithelial barrier function and host defensin peptide (beta-defensin 3) was studied by measuring the mRNA expression level of tight junction protein (occludin) and beta-defensin 3 in MODE-K cells. The strain RW1 showed a considerable potential to survive in simulated gastrointestinal environmental conditions, low pH and high bile salt concentrations along with good adhesion to MODE-K cell line. Pathogenic bacterial growth and their adhesion to MODE-K cell line were significantly inhibited by the strain RW1. Real-time PCR analyses demonstrated that the strain RW1 inhibited Salmonella-induced pro-inflammatory cytokines (IL-6, IL-8 and IL-1β) production and reinforced the expression of tight junction protein (occludin). The strain RW1 did not induce mRNA expression of beta-defensin 3.
Based on in vitro results, the strain RW1 has the potential to be used as a probiotic supplement in dogs. However, further study involving in vivo health effects is needed.
Antibiotics have many side effects and nowadays the probiotics are considered as a potential alternative to antibiotics. This study evaluates the probiotic potential of dog isolated L. plantarum strain RW1 to use it as a dietary supplement in dogs feeding to control infectious diseases.
评估从健康犬粪便中分离出的植物乳杆菌 RW1 菌株作为犬用膳食补充剂的益生菌潜力。
本研究旨在评估从犬粪便中分离出的植物乳杆菌 RW1 菌株的益生菌潜力。经传统方法和 16S rRNA 测序确认后，体外筛选 RW1 菌株在模拟胃肠道条件、低 pH 值、胆汁盐和黏附肠道上皮组织中的存活率，以及通过测量沙门氏菌感染的 MODE-K 细胞中白细胞介素 6 (IL-6)、白细胞介素 8 (IL-8)和白细胞介素 1β (IL-1β)的 mRNA 表达水平，评估其对常见病原体的生长抑制作用和抗炎潜力。此外，通过测量模式-K 细胞中紧密连接蛋白（occludin）和β-防御素 3 的 mRNA 表达水平，研究其对上皮屏障功能和宿主防御肽（β-防御素 3）的影响。RW1 株在模拟胃肠道环境条件、低 pH 值和高胆汁盐浓度下具有相当的生存能力，并且与 MODE-K 细胞系具有良好的黏附性。RW1 株显著抑制了致病菌的生长及其对 MODE-K 细胞系的黏附。实时 PCR 分析表明，RW1 株抑制了沙门氏菌诱导的促炎细胞因子（IL-6、IL-8 和 IL-1β）的产生，并增强了紧密连接蛋白（occludin）的表达。RW1 株不会诱导β-防御素 3 的 mRNA 表达。
基于体外结果，RW1 株具有作为犬用益生菌补充剂的潜力。然而，还需要进一步的体内健康影响研究。
抗生素有许多副作用，如今益生菌被认为是抗生素的潜在替代品。本研究评估了从犬分离出的植物乳杆菌 RW1 菌株作为犬用膳食补充剂的益生菌潜力，以控制犬的传染病。
